Team:Stockholm/26 July 2010

From 2010.igem.org

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# 8µl  Amp (0,5%)
# 8µl  Amp (0,5%)
Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON
Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON
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==Nina==
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==Sequencing CPP transportan 10 version C==
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I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing.
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* 15 ul vector
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* 1.5 ul 10uM VR2 primer
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9: ASB003N X99
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40: ASB003N X98

Revision as of 23:50, 31 July 2010

Contents

Mimmi

LMWP C and N

Plasmid prep and glycerol stock

The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid


DNA concentrations

Sample DNA (ng/μl)
LMWP N 60
LMWP C 80

over-expression in pEX - remaking the comassie-gel

MIX:

  1. 4ml LB
  2. 40µl glycose (1%)
  3. 8µl Amp (0,5%)

Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON

Nina

Sequencing CPP transportan 10 version C

I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing.

  • 15 ul vector
  • 1.5 ul 10uM VR2 primer

9: ASB003N X99

40: ASB003N X98

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