"

From 2010.igem.org

Revision as of 01:25, 31 July 2010 (view source) Annlesn (Talk | contribs) ← Older edit		Revision as of 02:08, 31 July 2010 (view source) Annlesn (Talk | contribs) Newer edit →
Line 51:		Line 51:
	[[Media:MODIFIED_Miniprep_Protocol.pdf|MODIFIED Miniprep]]		[[Media:MODIFIED_Miniprep_Protocol.pdf|MODIFIED Miniprep]]
	*[[Media:General_Miniprep_Protocol.pdf|Miniprep]]		*[[Media:General_Miniprep_Protocol.pdf|Miniprep]]
		+
		+	[[Media:General_DNA_Digest_Protocol.pdf|Nanodrop]]
	[[Media:General_DNA_Digest_Protocol.pdf|DNA Digest]]		[[Media:General_DNA_Digest_Protocol.pdf|DNA Digest]]

---

Revision as of 02:08, 31 July 2010

General Outline for Biobrick Manipulations

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

This is the procedure we can perform in the ERB

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

MODIFIED Miniprep

• Miniprep

Nanodrop

DNA Digest

Running a Gel

DNA Purification

Ligation

• Ginko Bioworks Protocol
• Jeremy Minty Protocol

Group-Specific Protocols

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols