Team:UNIPV-Pavia/Calendar/July/settimana4

JULY: WEEK 4

July, 19th

TECAN test showed that no RFP was produced from our parts, so all parts are potentially correct! For this reason we decided to sequence:

• I14-1 (Forward)
• I16-1 (Forward)
• I17-1 (Forward)
• I18-1 (Forward)
• I19-1 (Forward)

We also sequenced:

• I74C5-2 (Forward and Reverse)
• I84C5-2 (Forward and Reverse)
• I12-2 (Forward and Reverse)

These samples were prepared for sequencing (DNA was essicated) and sent to BMR genomics.

Trasformation of RING into:

• BW25141 (pir+)
• BW25142 (pir116)
• BW23474 (pir116)
• DH5alpha
• MG1655

Cultures were plated on:

• BW2514: Cm 34ug/ml
• BW25142: Cm 34ug/ml
• BW234741: Cm 34ug/ml
• DH5alpha: Cm 12,5ug/ml
• MG1655: Cm 12,5ug/ml

July, 20th

BW23474 transformed with <partinfo>BBa_J72007</partinfo>

DH5alpha transformed with <partinfo>BBa_J72007</partinfo>

Results for plates incubated ON, 37° C:

• BW23474 (pir116): showed colonies
• BW25141 (pir+): showed colonies
• BW25142 (pir116): showed colonies
• DH5alpha: didn't show colonies
• MG1655: didn't show colonies (even if there were a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).

BW23474 transformed with RING	BW25141 transformed with RING	BW25142 transformed with RING
DH5alpha transformed with RING	MG1655 transformed with RING

Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.

July, 21st

July, 22nd

July, 23rd

July, 24th

July, 25th