Team:Michigan/Virus Surface Display

From 2010.igem.org

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{{Michigan Header}}
{{Michigan Header}}
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7/10/2010
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13:00
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• digest I719015 (T7 GFPmut3b) and I719005(T7 Promoter) with EcoR1 and Pst1.
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7/13/2010
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9:00
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• inoculate competent cell culture
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• harvest I719015, K145001, K117008, K117002, K103006 from registry
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7/15/2010
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4:30
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• streak plate INP NC sample
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7/18/2010
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5:00
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• miniprep INP NC sample
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7/21/2010
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6:00
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• digest INP NC with EcoRI and SpeI
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7/23/2010
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9:00
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• gel electrophoresis of all biobricks
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[[Image:biobrickgel.jpg|300px]]
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[[Image:ladder.jpg|100px]]
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lane 1 - ladder
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lane 2 - INP NC sample 1 (K265008)
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lane 3 - INP NC sample 2 (K265008)
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lane 4 - T7 GFPmut3b (I719015)
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lane 5 - T7 Promoter (I719005)
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lane 6 - T7 RNA polymerase (K145001)
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lane 7 - pLsrA-YFP (K117008)
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lane 8 - LsrA promoter (K117002)
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lane 9 - OmpA (K103006)
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7/25/2010
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5:00
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• aliquot INP samples and primers for sequencing

Revision as of 00:06, 28 July 2010


Michigan Header





7/10/2010 13:00

• digest I719015 (T7 GFPmut3b) and I719005(T7 Promoter) with EcoR1 and Pst1.


7/13/2010 9:00

• inoculate competent cell culture

• harvest I719015, K145001, K117008, K117002, K103006 from registry


7/15/2010 4:30

• streak plate INP NC sample


7/18/2010 5:00

• miniprep INP NC sample


7/21/2010 6:00

• digest INP NC with EcoRI and SpeI


7/23/2010 9:00

• gel electrophoresis of all biobricks

Biobrickgel.jpg Ladder.jpg

lane 1 - ladder

lane 2 - INP NC sample 1 (K265008)

lane 3 - INP NC sample 2 (K265008)

lane 4 - T7 GFPmut3b (I719015)

lane 5 - T7 Promoter (I719005)

lane 6 - T7 RNA polymerase (K145001)

lane 7 - pLsrA-YFP (K117008)

lane 8 - LsrA promoter (K117002)

lane 9 - OmpA (K103006)


7/25/2010 5:00

• aliquot INP samples and primers for sequencing

Retrieved from "http://2010.igem.org/Team:Michigan/Virus_Surface_Display"