Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

• Adapt pH
• Agarose Gel
• Agar plates production
• Autoclave
• Competence Asaia --> follow protocol from Favia (more details in lab book, protocol: maybe Effie?)
• Electrophoresis
• Growing Asaia
• Ligation
• Measuring DNA concentration using NanoDrop
• Miniprep --> use Miniprep Kit (Protocol included in kit)
• Optical Density (OD)
• PCR (using the Roche Expand High Fidelity PCR System dNTPack
• PCR (using iProof BIORAD)
• Purification --> use Purification Kit (Protocol included in kit)
• Transformation of competent cells
• UV Imaging
• Using the plate reader (Solange or Stefano)
• Digestion

Media Recipes

• LB medium
• LB agar medium
• Gly medium
• Gly agar medium
• SOC medium

Syntax

Convention if you want to upload a new protocol. When uploading a file it please rename it in the following way:

 EPFL_prot_yourProcedureName.yourExtension