"
Team:Harvard/vectors
From 2010.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
<p>Our goal is the creation of an "iGarden" with the biobrick standard and in order for such a concept to work, we must get our constructs into plants. We used agrobacterium-mediated transformation, first in arabidopsis, then in strawberries. </p> | <p>Our goal is the creation of an "iGarden" with the biobrick standard and in order for such a concept to work, we must get our constructs into plants. We used agrobacterium-mediated transformation, first in arabidopsis, then in strawberries. </p> | ||
| - | <p>To do so, we created a set of <a href="vectors.html">vectors</a> compatible with the biobrick standard. To express our constucts - such as <a href="../flavor | + | <p>To do so, we created a set of <a href="vectors.html">vectors</a> compatible with the biobrick standard. To express our constucts - such as <a href="2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="2010.igem.org/Team:Harvard/fence">fence</a> mechanisms, we cloned the constucts into the agrobacterium vectors with E. coli as the chassis, then transformed into agrobacteria via electroporation, then innoculated our plants with the contruct containing agrobacteria. </p> |
<div> | <div> | ||
<img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"> | <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"> | ||
Revision as of 14:46, 27 July 2010
abstract lab notebook agrobacteria and plant engineering meet the vectors plant transformation parts & primers references
abstract
Our goal is the creation of an "iGarden" with the biobrick standard and in order for such a concept to work, we must get our constructs into plants. We used agrobacterium-mediated transformation, first in arabidopsis, then in strawberries.
To do so, we created a set of vectors compatible with the biobrick standard. To express our constucts - such as flavor elements, allergen knockdown, pigment accumulation, or fence mechanisms, we cloned the constucts into the agrobacterium vectors with E. coli as the chassis, then transformed into agrobacteria via electroporation, then innoculated our plants with the contruct containing agrobacteria.
