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Team:EPF Lausanne/Labbook protocols
From 2010.igem.org
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* Miniprep --> use Miniprep Kit | * Miniprep --> use Miniprep Kit | ||
*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)] | *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)] | ||
| - | *[https://static.igem.org/mediawiki/2010/ | + | *[https://static.igem.org/mediawiki/2010/5/54/Protocol_pcr_roche.pdf PCR (using the Roche Expand High Fidelity PCR System dNTPack ] |
*[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR (using iProof BIORAD)] | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR (using iProof BIORAD)] | ||
* Purification --> use Purification Kit | * Purification --> use Purification Kit | ||
Revision as of 14:29, 27 July 2010
Lab Protocols
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.
- Adapt pH
- Agarose Gel
- Agar plates production
- Autoclave
- Competence Asaia (Nadine)
- Electrophoresis
- Growing Asaia (Effie)
- Ligation
- Measuring DNA concentration using NanoDrop
- Miniprep --> use Miniprep Kit
- Optical Density (OD)
- PCR (using the Roche Expand High Fidelity PCR System dNTPack
- PCR (using iProof BIORAD)
- Purification --> use Purification Kit
- Transformation of competent cells
- UV Imaging
- Using the plate reader (Solange or Stefano)
- Digestion
Media Recipes
Syntax
Convention if you want to upload a new protocol. When uploading a file it please rename it in the following way:
EPFL_prot_yourProcedureName.yourExtension
