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Team:TU Delft/22 July 2010 content
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| - | A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will cut | + | A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will do a plasmid isolation with the cultures of lane 2, 4, 6, 7, 9 and 14. We will cut the isolated plasmids with various restriction enzymes and analyze the digestion products on gel. |
Revision as of 10:25, 26 July 2010
Alkane degradation
There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.
| # | Description | Expected length (bp) |
| 1 | [SmartLadder] (5 μL) | n/a |
| 2-4 | 007T | 1616 |
| 5-6 | 008T | 551 |
| 7-9 | 009T | 560 |
| 10-12 | 010T | 1657 |
| 13-15 | 017T | 1130 |
| 16 | 018T | 1874 |
| 17 | Red colony | n/a |
A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will do a plasmid isolation with the cultures of lane 2, 4, 6, 7, 9 and 14. We will cut the isolated plasmids with various restriction enzymes and analyze the digestion products on gel.
