Team:UNIPV-Pavia/Calendar/July/settimana3

From 2010.igem.org

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==July, 16th==
==July, 16th==
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[[Image:UNIPV10_screening_ligations_I11-I16.jpg|thumb|300px|center|Screening ligations I11-I16]]
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|[[Image:UNIPV10_screening_ligations_I11-I16.jpg|thumb|300px|center|Screening ligations I11-I16]] || [[Image:UNIPV10_creening_ligations_I17-I19_I7-8-104C5.jpg|thumb|300px|center|Screening ligations I17-I19, I7/I8/I104C5]]
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[[Image:UNIPV10_creening_ligations_I17-I19_I7-8-104C5.jpg|thumb|300px|center|Screening ligations I17-I19, I7/I8/I104C5]]
 
Since it's impossible to isolate the CRIM plasmid <partinfo>BBa_J72007</partinfo> we created a pseudo CRIM plasmid to check that not pir strains aren't able to propagate it.
Since it's impossible to isolate the CRIM plasmid <partinfo>BBa_J72007</partinfo> we created a pseudo CRIM plasmid to check that not pir strains aren't able to propagate it.

Revision as of 12:15, 24 July 2010

JULY: WEEK 3



July, 12th

Gel run/cut of phasins amplified via PCR

July, 13th

July, 14th

Quality control for HELPER and CRIM plasmids

July, 15th

Quality control for pBHR68 and CRIM plasmids

July, 16th

Screening ligations I11-I16
Screening ligations I17-I19, I7/I8/I104C5


Since it's impossible to isolate the CRIM plasmid <partinfo>BBa_J72007</partinfo> we created a pseudo CRIM plasmid to check that not pir strains aren't able to propagate it. We called it "THE RING" (RING) and we built it from our I5 (Cm resistance - <partinfo>BBa_P1004</partinfo> - + TT - <partinfo>BBa_B0015</partinfo> - + R6K ori - <partinfo>BBa_J61001</partinfo>) in <partinfo>pSB1A2</partinfo>:

  • Digestion of I5 XbaI-PstI
  • Gel extraction
  • Quantification with Nanodrop (15ng/ul)
  • Ligation of X and P sites each other (used 2ul of DNA)


Calendar

July

week 1

week 2

week 3

week 4

week 5