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Team:EPF Lausanne/Calendar/0713
From 2010.igem.org
(Difference between revisions)
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| - | + | *Chemical competence for E.Coli DB3.1 (step : Day 4) -> stored at -80°C | |
| + | *Preparation of GLY Medium / LB Medium / SOC Medium / TAE 1X / TAE 0.5X | ||
| + | *Run a gel for the PCR -> failed (mix of two different kits) | ||
| + | *Preparation of plates (GLY Agar / LB+Kan Agar / LB+Amp Agar) | ||
| + | *Learned to autoclave | ||
| + | *Restarted PCR (Asaia ORI + primers) | ||
| + | *Plated Asaia (O/N culture) | ||
| + | *Choice of Antibiotics Biobricks resistance (Kan / Amp / Tet / Chl) from the iGem Kit and preparation of them (streak out) | ||
| + | *Transformation of E.coli DB3.1 with pUC19 to check its competence | ||
| + | *Transformation of E.coli DH5 with p1003 (Kan) / p1002 (Amp) / p1004 (Chl) / p1005 (Tet) | ||
| + | *E.coli DB3.1 and DH5 were plated on LB+Amp Agar plates (Kan was plated on LB+Kan Agar plate) | ||
| + | *Ordered material | ||
| + | *Wiki brainstorming | ||
| + | *Protocols printing and organization | ||
</div> | </div> | ||
Latest revision as of 18:51, 23 July 2010
13 July 2010
- Chemical competence for E.Coli DB3.1 (step : Day 4) -> stored at -80°C
- Preparation of GLY Medium / LB Medium / SOC Medium / TAE 1X / TAE 0.5X
- Run a gel for the PCR -> failed (mix of two different kits)
- Preparation of plates (GLY Agar / LB+Kan Agar / LB+Amp Agar)
- Learned to autoclave
- Restarted PCR (Asaia ORI + primers)
- Plated Asaia (O/N culture)
- Choice of Antibiotics Biobricks resistance (Kan / Amp / Tet / Chl) from the iGem Kit and preparation of them (streak out)
- Transformation of E.coli DB3.1 with pUC19 to check its competence
- Transformation of E.coli DH5 with p1003 (Kan) / p1002 (Amp) / p1004 (Chl) / p1005 (Tet)
- E.coli DB3.1 and DH5 were plated on LB+Amp Agar plates (Kan was plated on LB+Kan Agar plate)
- Ordered material
- Wiki brainstorming
- Protocols printing and organization
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