From 2010.igem.org
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| - | |[[Image:UNIPV10_BW23474_J72007.jpg|thumb| 300px|center|BW23474 transformed with <partinfo>BBa_J72007</partinfo>]] || [[Image:UNIPV10_DH5alpha_J72007.jpg|thumb| 300px|center|DH5alpha transformed with <partinfo>BBa_J72007</partinfo>]] | + | |[[Image:UNIPV10_BW23474_J72007.jpg|thumb| 200px|center|BW23474 transformed with <partinfo>BBa_J72007</partinfo>]] || [[Image:UNIPV10_DH5alpha_J72007.jpg|thumb| 200px|center|DH5alpha transformed with <partinfo>BBa_J72007</partinfo>]] |
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| - | |[[Image:UNIPV10_BW23474_RING.jpg|thumb|300px|center|BW23474 transformed with RING]] || [[Image:UNIPV10_BW25141_RING.jpg|thumb|300px|center|BW25141 transformed with RING]] | + | |[[Image:UNIPV10_BW23474_RING.jpg|thumb|200px|center|BW23474 transformed with RING]] || [[Image:UNIPV10_BW25141_RING.jpg|thumb|200px|center|BW25141 transformed with RING]] |
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| - | |[[Image:UNIPV10_BW25142_RING.jpg|thumb|300px|center|BW25142 transformed with RING]] || [[Image:UNIPV10_DH5alpha_RING.jpg|thumb|300px|center|DH5alpha transformed with RING]] | + | |[[Image:UNIPV10_BW25142_RING.jpg|thumb|200px|center|BW25142 transformed with RING]] || [[Image:UNIPV10_DH5alpha_RING.jpg|thumb|200px|center|DH5alpha transformed with RING]] |
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| - | |colspan="2"|[[Image:UNIPV10_MG1655_RING.jpg|thumb|300px|center|MG1655 transformed with RING]] | + | |colspan="2"|[[Image:UNIPV10_MG1655_RING.jpg|thumb|200px|center|MG1655 transformed with RING]] |
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Revision as of 20:59, 21 July 2010
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JULY: WEEK 4
July, 19th
Trasformation of RING into:
- BW25141 (pir+)
- BW25142 (pir116)
- BW23474 (pir116)
- DH5alpha
- MG1655
Cultures were plated on:
- BW2514: Cm 34ng/ml
- BW25142: Cm 3nug/ml
- BW234741: Cm 34ng/ml
- DH5alpha: Cm 12,5ng/ml
- MG1655: Cm 12,5ng/ml
July, 20th
 BW23474 transformed with <partinfo>BBa_J72007</partinfo> |  DH5alpha transformed with <partinfo>BBa_J72007</partinfo> |
Results for plates incubated ON, 37° C:
- BW23474 (pir116): showed colonies
- BW25141 (pir+): showed colonies
- BW25142 (pir116): showed colonies
- DH5alpha: didn't show colonies
- MG1655: didn't show colonies (even if there were a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).
 BW23474 transformed with RING |  BW25141 transformed with RING |
 BW25142 transformed with RING |  DH5alpha transformed with RING |
 MG1655 transformed with RING |
Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.
July, 21st
July, 22nd
July, 23rd
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