Team:UNIPV-Pavia/Calendar/July/settimana4

From 2010.igem.org

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==July, 20th==
==July, 20th==
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{|align="center"
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|-
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|[[Image:UNIPV10_BW23474_J72007.jpg|thumb|300px|center|BW23474 transformed with <partinfo>BBa_J72007</partinfo>]] ||  [[Image:UNIPV10_DH5alpha_J72007.jpg|thumb|300px|center|DH5alpha transformed with <partinfo>BBa_J72007</partinfo>]]
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|-
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Results for plates incubated ON, 37° C:
Results for plates incubated ON, 37° C:
*BW23474 (pir116): showed colonies
*BW23474 (pir116): showed colonies
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*DH5alpha: didn't show colonies
*DH5alpha: didn't show colonies
*MG1655: didn't show colonies (even if there were a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).
*MG1655: didn't show colonies (even if there were a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).
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{|align="center"
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|-
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|[[Image:UNIPV10_BW23474_RING.jpg|thumb|300px|center|BW23474 transformed with RING]] || [[Image:UNIPV10_BW25141_RING.jpg|thumb|300px|center|BW25141 transformed with RING]]
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|-
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|[[Image:UNIPV10_BW25142_RING.jpg|thumb|300px|center|BW25142 transformed with RING]] || [[Image:UNIPV10_DH5alpha_RING.jpg|thumb|300px|center|DH5alpha transformed with RING]]
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|-
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|colspan="2"|[[Image:UNIPV10_MG1655_RING.jpg|thumb|300px|center|MG1655 transformed with RING]]
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Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.
Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.

Revision as of 20:58, 21 July 2010
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JULY: WEEK 4


July, 19th

Trasformation of RING into:

  • BW25141 (pir+)
  • BW25142 (pir116)
  • BW23474 (pir116)
  • DH5alpha
  • MG1655

Cultures were plated on:

  • BW2514: Cm 34ng/ml
  • BW25142: Cm 3nug/ml
  • BW234741: Cm 34ng/ml
  • DH5alpha: Cm 12,5ng/ml
  • MG1655: Cm 12,5ng/ml

July, 20th

BW23474 transformed with <partinfo>BBa_J72007</partinfo>
DH5alpha transformed with <partinfo>BBa_J72007</partinfo>

Results for plates incubated ON, 37° C:

  • BW23474 (pir116): showed colonies
  • BW25141 (pir+): showed colonies
  • BW25142 (pir116): showed colonies
  • DH5alpha: didn't show colonies
  • MG1655: didn't show colonies (even if there were a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).
BW23474 transformed with RING
BW25141 transformed with RING
BW25142 transformed with RING
DH5alpha transformed with RING
MG1655 transformed with RING

Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.

July, 21st

July, 22nd

July, 23rd

Calendar

July

week 1

week 2

week 3

week 4

week 5

Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana4"