From 2010.igem.org
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| | Cultures were plated on: | | Cultures were plated on: |
| - | *BW2514: Cm 34ug/ml | + | *BW2514: Cm 34ng/ml |
| - | *BW25142: Cm 34ug/ml | + | *BW25142: Cm 3nug/ml |
| - | *BW234741: Cm 34ug/ml | + | *BW234741: Cm 34ng/ml |
| - | *DH5alpha: Cm 12,5ug/ml | + | *DH5alpha: Cm 12,5ng/ml |
| - | *MG1655: Cm 12,5ug/ml | + | *MG1655: Cm 12,5ng/ml |
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| | ==July, 20th== | | ==July, 20th== |
Revision as of 14:27, 20 July 2010
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JULY: WEEK 4
July, 19th
Trasformation of RING into:
- BW25141 (pir+)
- BW25142 (pir116)
- BW23474 (pir116)
- DH5alpha
- MG1655
Cultures were plated on:
- BW2514: Cm 34ng/ml
- BW25142: Cm 3nug/ml
- BW234741: Cm 34ng/ml
- DH5alpha: Cm 12,5ng/ml
- MG1655: Cm 12,5ng/ml
July, 20th
Results for plates incubated ON, 37° C:
- BW23474 (pir116): showed colonies
- BW25141 (pir+): showed colonies
- BW25142 (pir116): showed colonies
- DH5alpha: didn't show colonies
- MG1655: didn't show colonies (even if there were a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).
Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.
July, 21st
July, 22nd
July, 23rd
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