Team:UNIPV-Pavia/Calendar/July/settimana4

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Revision as of 14:18, 20 July 2010

JULY: WEEK 4

July, 19th

Trasformation of RING into:

BW25141 (pir+)
BW25142 (pir116)
BW23474 (pir116)
DH5alpha
MG1655

Cultures were plated on:

BW2514: Cm 34ug/ml
BW25142: Cm 34ug/ml
BW234741: Cm 34ug/ml
DH5alpha: Cm 12,5ug/ml
MG1655: Cm 12,5ug/ml

July, 20th

Results for plates incubated ON, 37°:

BW23474 (pir116): showed colonies
BW25141 (pir+): showed colonies
BW25142 (pir116): showed colonies
DH5alpha: didn't show colonies
MG1655: didn't show colonies (even if there are a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).

Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.

July, 21st

July, 22nd

July, 23rd

week 4

week 5

@@ Line 33: / Line 33: @@
 *BW25142 (pir116): showed colonies
 *DH5alpha: didn't show colonies
-*MG1655: didn't show colonies
+*MG1655: didn't show colonies (even if there are a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).
+Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.
 ==July, 21st==