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Team:Stanford/Notebook/Lab Work/Week 3
From 2010.igem.org
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==7/6 Tuesday== | ==7/6 Tuesday== | ||
| + | ''Greg'': | ||
| + | *During weekly meeting got up to speed with what the team’s been doing while I was away | ||
| + | *Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight: | ||
| + | |||
| + | |||
| + | {| class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | ! Function !! Part # !! Distribution Location !! Resistance | ||
| + | |- | ||
| + | | ribosome binding site || B0032 || P1 2I || Amp | ||
| + | |- | ||
| + | | forward double terminator || B0015 || P1 23L || Amp + Kan | ||
| + | |- | ||
| + | | bidirectional double terminator || B0014 || P2 24C || Amp + Kan | ||
| + | |- | ||
| + | | medium constitutive promoter || J23107 || P1 20A || Amp | ||
| + | |- | ||
| + | | strong constitutive promoter || J23100 || P1 18C || Amp | ||
| + | |- | ||
| + | | plasmid backbone || pSB1K3 || P1 5A || Kan | ||
| + | |} | ||
==7/8 Thursday== | ==7/8 Thursday== | ||
Revision as of 00:08, 20 July 2010
|
Spring: Brainstorming | Spring Meetings
Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries
|
7/5 Monday
Planning Meeting Notes
Agenda
- Design Updates
- Analog Comparator (Chris & Alex)
- Redundancy between the two proposals
- Degree of phosporylation
- Dynamic range and mechanisms
- Digital Comparator (Francisco & Karina)
- RSID; standardizing the design
- Introducing a PoPs signal output
- Characteristics between the two Comparators
- What makes the two different?
- Applications
- Vaginal Infections/Preterm Birth
- is it really a ratio?
- Other ideas: Targeting Cancer Cells, oscillators
- Administrative Details
- Ordering Stuff: Oligos ordering sheet, Digestion and Gel Extraction Kits?
- Stipend?
- Sponsorship Updates
- MDV Presentation
Goals For the Week
- Chris & Alex
- need to make 50x TAE
- complete plasmids as far can get w/o kinase and phosphotase
- order DNA sometime this week
- Francisco & Karina
- Run orginal and new designs by Christina
- design standard sRNA designs and place order by the end of the week
7/6 Tuesday
Greg:
- During weekly meeting got up to speed with what the team’s been doing while I was away
- Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight:
| Function | Part # | Distribution Location | Resistance |
|---|---|---|---|
| ribosome binding site | B0032 | P1 2I | Amp |
| forward double terminator | B0015 | P1 23L | Amp + Kan |
| bidirectional double terminator | B0014 | P2 24C | Amp + Kan |
| medium constitutive promoter | J23107 | P1 20A | Amp |
| strong constitutive promoter | J23100 | P1 18C | Amp |
| plasmid backbone | pSB1K3 | P1 5A | Kan |
