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Team:TU Delft/16 July 2010 content
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| - | The [[Team:TU_Delft/protocols/birnboim_plasmid_isolation Birnboim method ]] was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. | + | The [[Team:TU_Delft/protocols/birnboim_plasmid_isolation|Birnboim method]] was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. |
Revision as of 22:40, 19 July 2010
Contents |
Lab work
Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing
The colony PCR of yesterday was put on1% agarose gel
We harvested the 1 mL bacterial cells of possible correct transformations. The pellets were stored at -20 °C. We used 3 mL of the bacterial cells to make -80 °C stocks.
Characterization of Anderson RBS sequences
Fluorescence measurements Attempt #2
Data analysis to follow shortly (good stuff!)
Assembly of reference construct
The Birnboim method was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures.
