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Team:Stanford/Notebook/Lab Work/Week 1
From 2010.igem.org
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==Monday== | ==Monday== | ||
'''General Meeting''' | '''General Meeting''' | ||
| - | + | Wiki stuff | |
| - | + | *Everyone should sign up on 2010.igem.org website | |
| - | + | **Take advantage of the new calendar system | |
| - | + | **Need to transfer current website over | |
| - | + | *Current wiki needs a new vision | |
| - | + | **Francisco + Alex assembling wiki committee | |
| - | + | Transform the first wave of cells | |
| - | + | *Pick colonies | |
| - | + | *Colony PCR | |
| - | + | *Sequence parts to validate | |
| - | + | **Use the Smolke sequencing network | |
| - | + | Characterize promoters | |
| - | + | *Digest, ligate | |
| - | + | *Need to acquire enzymes | |
| - | + | *Need an PO account. | |
| - | + | **Email Jeanne | |
| - | + | Get competent cells | |
| - | + | Getting DNA | |
| - | + | *Assembly PCR | |
| - | + | *PCR from host cells | |
| - | + | *Synthesize de novo | |
| - | + | RNA team | |
| - | + | *Use RNAstructure | |
| - | + | **Maybe use ViennaRNA or dinamelt | |
| - | + | *Talk to Arkin and Berkeley Postdocs | |
| - | + | *Finalize sRNA library | |
| - | + | *9kb plasmid | |
| - | + | Meeting scheudle | |
| - | + | *Monday morning is good for logistics | |
| - | + | *Group meetings for presentations | |
| - | + | **One person presents their work in the context of everything else | |
| - | + | *Journal club | |
| - | + | *Karina will set up a doodle | |
| - | + | ||
</div> | </div> | ||
Revision as of 22:39, 19 July 2010
|
Spring: Brainstorming | Spring Meetings
Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries
|
Monday
General Meeting Wiki stuff
- Everyone should sign up on 2010.igem.org website
- Take advantage of the new calendar system
- Need to transfer current website over
- Current wiki needs a new vision
- Francisco + Alex assembling wiki committee
Transform the first wave of cells
- Pick colonies
- Colony PCR
- Sequence parts to validate
- Use the Smolke sequencing network
Characterize promoters
- Digest, ligate
- Need to acquire enzymes
- Need an PO account.
- Email Jeanne
Get competent cells Getting DNA
- Assembly PCR
- PCR from host cells
- Synthesize de novo
RNA team
- Use RNAstructure
- Maybe use ViennaRNA or dinamelt
- Talk to Arkin and Berkeley Postdocs
- Finalize sRNA library
- 9kb plasmid
Meeting scheudle
- Monday morning is good for logistics
- Group meetings for presentations
- One person presents their work in the context of everything else
- Journal club
- Karina will set up a doodle
