"
Team:TU Delft/16 July 2010 content
From 2010.igem.org
(Difference between revisions)
(→Lab work) |
(→Characterization of Anderson RBS sequences) |
||
| Line 8: | Line 8: | ||
==Characterization of Anderson RBS sequences== | ==Characterization of Anderson RBS sequences== | ||
| + | <h5>Fluorescence measurements Attempt #2</h5> | ||
| + | Data analysis to follow shortly (good stuff!) | ||
| + | |||
| + | <h5>Assembly of reference construct</h5> | ||
| + | The Birnboim method was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. | ||
Revision as of 22:39, 19 July 2010
Contents |
Lab work
Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing
The colony PCR of yesterday was put on1% agarose gel
We harvested the 1 mL bacterial cells of possible correct transformations. The pellets were stored at -20 °C. We used 3 mL of the bacterial cells to make -80 °C stocks.
Characterization of Anderson RBS sequences
Fluorescence measurements Attempt #2
Data analysis to follow shortly (good stuff!)
Assembly of reference construct
The Birnboim method was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures.
