Team:Stanford/NotebookPage/14 July 2010
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Chrisvanlang (Talk | contribs) (New page: {{Template:Stanford_Main_Menu_0910}} {{Template: Stanford Notebook Calendar}} <div style="width:720px;text-align:justify"> ==Cool Links== *[http://blogs.discovermagazine.com/notrocketscie...) |
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*[http://blogs.discovermagazine.com/notrocketscience/2010/07/12/genes-from-arctic-bacteria-used-to-create-new-vaccines/ genes-from-arctic-bacteria-used-to-create-new-vaccines] | *[http://blogs.discovermagazine.com/notrocketscience/2010/07/12/genes-from-arctic-bacteria-used-to-create-new-vaccines/ genes-from-arctic-bacteria-used-to-create-new-vaccines] | ||
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+ | ==Laura's Lab notebook== | ||
+ | *plates restreaked yesterday look good- bacteria grew well, some individual colonies | ||
+ | *helped Francisco with minipreps (Promega kit) of : terminator, GFP, RFP | ||
+ | **Francisco set up liquid cultures yesterday afternoon | ||
+ | *Francisco poured, ran 0.8% gel | ||
+ | **yesterday's digestions, with today's minipreps as controls | ||
+ | *gel extraction: (I cut out bands, Francisco and Karina extracted) | ||
+ | {| | ||
+ | |part || tube mass (g) || tube + gel (g) || gel (g) || volume QG (uL) | ||
+ | |- | ||
+ | |terminator || 1.03 || 1.14 || 0.11 || 330 | ||
+ | |- | ||
+ | |RFP || 1.11 || 1.18 || 0.07 || 210 | ||
+ | |- | ||
+ | |GFP || 1.03 || 1.13 || 0.10 || 300 | ||
+ | |} |
Latest revision as of 21:54, 19 July 2010
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Cool Links
- [http://blogs.discovermagazine.com/notrocketscience/2010/07/12/genes-from-arctic-bacteria-used-to-create-new-vaccines/ genes-from-arctic-bacteria-used-to-create-new-vaccines]
Laura's Lab notebook
- plates restreaked yesterday look good- bacteria grew well, some individual colonies
- helped Francisco with minipreps (Promega kit) of : terminator, GFP, RFP
- Francisco set up liquid cultures yesterday afternoon
- Francisco poured, ran 0.8% gel
- yesterday's digestions, with today's minipreps as controls
- gel extraction: (I cut out bands, Francisco and Karina extracted)
part | tube mass (g) | tube + gel (g) | gel (g) | volume QG (uL) |
terminator | 1.03 | 1.14 | 0.11 | 330 |
RFP | 1.11 | 1.18 | 0.07 | 210 |
GFP | 1.03 | 1.13 | 0.10 | 300 |