Team:Stanford/Notebook/Lab Work/Week 1
From 2010.igem.org
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(→June 28) |
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=====June 28===== | =====June 28===== | ||
+ | General Meeting | ||
+ | *Wiki stuff | ||
+ | **Everyone should sign up on 2010.igem.org website | ||
+ | ***Take advantage of the new calendar system | ||
+ | ***Need to transfer current website over | ||
+ | **Current wiki needs a new vision | ||
+ | ***Francisco + Alex assembling wiki committee | ||
+ | *Transform the first wave of cells | ||
+ | **Pick colonies | ||
+ | **Colony PCR | ||
+ | **Sequence parts to validate | ||
+ | ***Use the Smolke sequencing network | ||
+ | *Characterize promoters | ||
+ | **Digest, ligate | ||
+ | **Need to acquire enzymes | ||
+ | **Need an PO account. | ||
+ | ***Email Jeanne | ||
+ | *Get competent cells | ||
+ | *Getting DNA | ||
+ | **Assembly PCR | ||
+ | **PCR from host cells | ||
+ | **Synthesize de novo | ||
+ | *RNA team | ||
+ | **Use RNAstructure | ||
+ | ***Maybe use ViennaRNA or dinamelt | ||
+ | **Talk to Arkin and Berkeley Postdocs | ||
+ | **Finalize sRNA library | ||
+ | **9kb plasmid | ||
+ | * | ||
+ | *Meeting scheudle | ||
+ | **Monday morning is good for logistics | ||
+ | **Group meetings for presentations | ||
+ | ***One person presents their work in the context of everything else | ||
+ | **Journal club | ||
+ | **Karina will set up a doodle |
Revision as of 21:26, 19 July 2010
June 28
General Meeting
- Wiki stuff
- Everyone should sign up on 2010.igem.org website
- Take advantage of the new calendar system
- Need to transfer current website over
- Current wiki needs a new vision
- Francisco + Alex assembling wiki committee
- Everyone should sign up on 2010.igem.org website
- Transform the first wave of cells
- Pick colonies
- Colony PCR
- Sequence parts to validate
- Use the Smolke sequencing network
- Characterize promoters
- Digest, ligate
- Need to acquire enzymes
- Need an PO account.
- Email Jeanne
- Get competent cells
- Getting DNA
- Assembly PCR
- PCR from host cells
- Synthesize de novo
- RNA team
- Use RNAstructure
- Maybe use ViennaRNA or dinamelt
- Talk to Arkin and Berkeley Postdocs
- Finalize sRNA library
- 9kb plasmid
- Use RNAstructure
- Meeting scheudle
- Monday morning is good for logistics
- Group meetings for presentations
- One person presents their work in the context of everything else
- Journal club
- Karina will set up a doodle