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Team:Stanford/NotebookPage/9 July 2010
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Karpadillo (Talk | contribs) (→Karina's Notebook) |
Karpadillo (Talk | contribs) (→Karina's Notebook) |
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GFP: E0040 (cut at S and P) <br/> | GFP: E0040 (cut at S and P) <br/> | ||
RFP: E1010 (cut at S and P) <br/> | RFP: E1010 (cut at S and P) <br/> | ||
| - | Term: B1006 (cut at X and P) | + | Term: B1006 (cut at X and P) <br/><br/> |
'''Recipe''' <br/> | '''Recipe''' <br/> | ||
| Line 17: | Line 17: | ||
5.0 uL NEB buffer 2<br/> | 5.0 uL NEB buffer 2<br/> | ||
5 uL BSA (10x) <br/> | 5 uL BSA (10x) <br/> | ||
| - | Sterile H20 to fill up to 50 uL | + | Sterile H20 to fill up to 50 uL<br/><br/> |
| + | |||
| + | Mix and put 37º waterbath for 2 or more hours. <br/><br/> | ||
| + | |||
| + | '''Making Gel'''<br/> | ||
| + | To make 50 mL of 0.8% agarose gel, add: <br/> | ||
| + | .4 g agarose <br/> | ||
| + | 50 mL TAE (1x)<br/> | ||
| + | 10 uL EtBr<br/><br/> | ||
Revision as of 19:01, 19 July 2010
[http://docs.google.com/present/edit?id=0Ae31et9w_tAhZGZyc3R2ejJfOGNnY2drMmZz&hl=en&authkey=CPHGzfIB Weekly Leader Presentation: Karina]
Karina's Notebook
Today's Goal: Digest GFP, RFP, and Terminators. Will do (GFP + T) and (RFP + T) ligations on monday.
Part #'s:
GFP: E0040 (cut at S and P)
RFP: E1010 (cut at S and P)
Term: B1006 (cut at X and P)
Recipe
12.0 uL DNA
1.0 uL each enzyme
5.0 uL NEB buffer 2
5 uL BSA (10x)
Sterile H20 to fill up to 50 uL
Mix and put 37º waterbath for 2 or more hours.
Making Gel
To make 50 mL of 0.8% agarose gel, add:
.4 g agarose
50 mL TAE (1x)
10 uL EtBr
