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Team:Stanford/NotebookPage/9 July 2010
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== Karina's Notebook == | == Karina's Notebook == | ||
| - | Today's Goal: Digest GFP, RFP, and Terminators. Will | + | Today's Goal: Digest GFP, RFP, and Terminators. Will do (GFP + T) and (RFP + T) ligations on monday. <br/><br/> |
'''Part #'s:''' <br/> | '''Part #'s:''' <br/> | ||
GFP: E0040 (cut at S and P) <br/> | GFP: E0040 (cut at S and P) <br/> | ||
Revision as of 18:57, 19 July 2010
[http://docs.google.com/present/edit?id=0Ae31et9w_tAhZGZyc3R2ejJfOGNnY2drMmZz&hl=en&authkey=CPHGzfIB Weekly Leader Presentation: Karina]
Karina's Notebook
Today's Goal: Digest GFP, RFP, and Terminators. Will do (GFP + T) and (RFP + T) ligations on monday.
Part #'s:
GFP: E0040 (cut at S and P)
RFP: E1010 (cut at S and P)
Term: B1006 (cut at X and P)
Recipe
12.0 uL DNA
1.0 uL each enzyme
5.0 uL NEB buffer 2
5 uL BSA (10x)
Sterile H20 to fill up to 50 uL
