Team:Newcastle/15 July 2010

From 2010.igem.org

(Difference between revisions)
(New page: ==Summary== Screening for lacI insert: (Miniprep) #Restriction digests #*Single digest with Pst1 #*double digests with Pst1 and Xba1 #Gel electrophoresis Set up liquid broth culture in ...)
(Summary)
Line 2: Line 2:
Screening for lacI insert:
Screening for lacI insert:
-
(Miniprep)
+
|(Miniprep)
#Restriction digests
#Restriction digests
#*Single digest with Pst1  
#*Single digest with Pst1  

Revision as of 10:51, 19 July 2010

Summary

Screening for lacI insert: |(Miniprep)

  1. Restriction digests
    • Single digest with Pst1
    • double digests with Pst1 and Xba1
  2. Gel electrophoresis

Set up liquid broth culture in LB