Team:TU Delft/9 June 2010 content
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We got the first colonies, and they're red! Hurray! | We got the first colonies, and they're red! Hurray! | ||
- | Yesterday we grew colonies containing the plasmids (with RFP). Today one colony from each plate was picked and cultivated in liquid as well as solid LB medium with the corresponding antibiotic to grow overnight. | + | Yesterday we grew colonies containing the plasmids (with RFP). Today one colony from each plate was picked and cultivated in liquid (4ml LB + 4μl antibiotic) as well as solid LB medium (+20μl antibiotic) with the corresponding antibiotic to grow overnight. |
Thias purified the PCR products. | Thias purified the PCR products. | ||
[[Image:09062010171.jpg|250px|thumb|left|Red colonies]] | [[Image:09062010171.jpg|250px|thumb|left|Red colonies]] |
Revision as of 08:44, 15 July 2010
Lab work
We got the first colonies, and they're red! Hurray!
Yesterday we grew colonies containing the plasmids (with RFP). Today one colony from each plate was picked and cultivated in liquid (4ml LB + 4μl antibiotic) as well as solid LB medium (+20μl antibiotic) with the corresponding antibiotic to grow overnight.
Thias purified the PCR products.