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Team:TU Delft/24 April 2010 content
From 2010.igem.org
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Split into 3 teams: | Split into 3 teams: | ||
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| + | '''Hydrocarbon degradation''' | ||
* Genes for enzymes that degrade various aromatic compounds are known | * Genes for enzymes that degrade various aromatic compounds are known | ||
* Maybe start with Benzoic acid & Alkenes? | * Maybe start with Benzoic acid & Alkenes? | ||
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* Be careful whether the intermediates may be toxic | * Be careful whether the intermediates may be toxic | ||
* Sugar metabolism is no longer necessary in E.coli | * Sugar metabolism is no longer necessary in E.coli | ||
| - | + | ||
| + | '''Salt tolerance''' | ||
* Give the other team a strain of E.coli that can handle salt. | * Give the other team a strain of E.coli that can handle salt. | ||
* Article: gene that helps in salt resistance (sea levels, NaCl) but function isn’t really understood | * Article: gene that helps in salt resistance (sea levels, NaCl) but function isn’t really understood | ||
* Biobrick 2009; not clear whether it works or not? | * Biobrick 2009; not clear whether it works or not? | ||
| - | + | ||
| + | '''Overcoming mass transfer''' | ||
* Adding an emulsifier to make the oil soluble? | * Adding an emulsifier to make the oil soluble? | ||
* Peptide production made of Leucine & Glutamic acid (paper) with an export mechanism | * Peptide production made of Leucine & Glutamic acid (paper) with an export mechanism | ||
* Production of hydrophilic/hydrophobic peptide | * Production of hydrophilic/hydrophobic peptide | ||
| - | + | ||
| + | '''Extra features''' | ||
* We are making allot of acetyl CoA, so why not produce something useful? | * We are making allot of acetyl CoA, so why not produce something useful? | ||
* Biofuel? Propanol/ethanol/biodiesel? There are some biobricks for this… but no Exporter? (Biobrick) | * Biofuel? Propanol/ethanol/biodiesel? There are some biobricks for this… but no Exporter? (Biobrick) | ||
* PHA/PHB | * PHA/PHB | ||
* What about working at higher temperatures? Ethanol vaporization | * What about working at higher temperatures? Ethanol vaporization | ||
| - | + | ||
| + | '''Other points''' | ||
* Money / many biobricks / lot of literature | * Money / many biobricks / lot of literature | ||
* Is E.coli the bug to go with? | * Is E.coli the bug to go with? | ||
Revision as of 12:41, 14 July 2010
Contents |
Session 8
Now it’s not if we like the idea anymore (otherwise it wouldn’t be a chosen idea), but how could we accomplish it? How feasible is it? What BioBricks do we expect to make with this?
1. Oil removal
- Hydrocarbon degradation
- Oil sand processing gives wastewater with allot of oil (bitumen) => Convert this wastewater into something valuable
- Salinity, pH, mass transfer problems
Split into 3 teams:
Hydrocarbon degradation
- Genes for enzymes that degrade various aromatic compounds are known
- Maybe start with Benzoic acid & Alkenes?
- Probably the most work of all the teams, but others could help once they are done
- Most likely an aerobic process (most likely none of the enzymes are oxygen sensitive)
- Be careful whether the intermediates may be toxic
- Sugar metabolism is no longer necessary in E.coli
Salt tolerance
- Give the other team a strain of E.coli that can handle salt.
- Article: gene that helps in salt resistance (sea levels, NaCl) but function isn’t really understood
- Biobrick 2009; not clear whether it works or not?
Overcoming mass transfer
- Adding an emulsifier to make the oil soluble?
- Peptide production made of Leucine & Glutamic acid (paper) with an export mechanism
- Production of hydrophilic/hydrophobic peptide
Extra features
- We are making allot of acetyl CoA, so why not produce something useful?
- Biofuel? Propanol/ethanol/biodiesel? There are some biobricks for this… but no Exporter? (Biobrick)
- PHA/PHB
- What about working at higher temperatures? Ethanol vaporization
Other points
- Money / many biobricks / lot of literature
- Is E.coli the bug to go with?
- We don’t necessarily have to put all 4 projects into one strain, but we can start with 4 and maybe if we have time we could try to.
- Good project that represents Delft (mass transfer etc.)
- There are no biobricks for hydrocarbon degradation!
Vote: Yes (8/8)
2. Algae bloom /nitrate reduction
- Fumarate reductase is the only difference between the aerobic and anaerobic paths?
- A mutated FNR can induce the nitrate consumption under aerobic conditions
- Biobrick for nitrite sensor
- E.coli would probably be outcompeted by the algae? Depends on the carbon source
- Some enzymes will be inhibited by oxygen
Vote: No (0/8)
3. Appetite inhibitor
- Caerulein
- Combination with circadian clock for times of production and non-production?
- Combination with cell differentiation for part of the MOs to produce (and maybe die), while the others don’t produce the peptide.
- Regulation is definitely necessary
- Couple it to blood sugar levels? Not really possible to sensor something in the blood from within the gut?
Vote: No (3/8)
4. Circadian Clocks / oscillators
- Oscillations are easy to come by
- Maybe too simple as a project on its own?
- Tool for another project?
- Hard to accurately predict the actual frequency; could only occur through true labwork
- Frequency would be dependent on the concentrations of the compounds as well as the growth rates for these also determine the degradation etc.
- How could you change affinity constants? (Kai system)
- Biobrick for Kai is already available, but how can you influence the frequency without trial-and-error?
- There is literature that could help make a model…
Vote: No (3/8)
