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Revision as of 16:00, 13 July 2010 (view source) Daye (Talk | contribs) (→BioBrick Discussion) ← Older edit		Revision as of 07:54, 14 July 2010 (view source) Yessa (Talk | contribs) (→Allocation of team members to cloning strategies:) Newer edit →
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	====Allocation of team members to cloning strategies:====		====Allocation of team members to cloning strategies:====
	*'''YneA''' - Phil, Rach, Deena, Younus		*'''YneA''' - Phil, Rach, Deena, Younus
-	*'''Swarming (2)''' - Harsh, Alan, Steve, Da
	*'''LacI''' - Phil, Younus, Rachel		*'''LacI''' - Phil, Younus, Rachel
		+	*'''Swarming (2)''' - Harsh, Alan, Steve, Da
	*'''Subtilin (2)''' - Harsh, Alan, Steve, Deena		*'''Subtilin (2)''' - Harsh, Alan, Steve, Deena
	*'''RocF''' - Harsh, Alan, Steve, Da		*'''RocF''' - Harsh, Alan, Steve, Da
-
-
	==Wiki==		==Wiki==

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Revision as of 07:54, 14 July 2010

• Informal meeting at the Walton Library [3.30 - 5.00pm]
• Chair: Da, Minutes: Younus

Contents 1 Cloning Strategies 1.1 5 parts to a Cloning Strategy: 1.2 Required Cloning Strategies 1.3 Allocation of team members to cloning strategies: 2 Wiki 2.1 Labbook 2.2 General: 3 UK iGEM Gathering at Newcastle - 20th/21st July 3.1 Presentation: 3.1.1 Introduction 3.1.2 General Outlook 3.1.3 BioBrick Discussion 3.1.4 Whats next... 4 Minideadlines 5 Action Points

Cloning Strategies

5 parts to a Cloning Strategy:

The cloning strategies for each biobrick should contain the following sections in this order:

1. Purpose and Justification
2. Modelling
3. Construction
4. Cloning and Integration
5. Testing and Characterising

Required Cloning Strategies

There are 8 cloning strategies which are required:

• SR1 - finished!
• YneA - biobrick nearly coming back - our most important biobrick!
• LacI - not sure how to test this biobrick
• 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
• 2 for Subtilin (immunity and production) - cloning strategies nearly finished
• RocF (need to use Gibson method) - not started

So only the cloning strategy for SR1 is finished. 7 more to go!

Allocation of team members to cloning strategies:

• YneA - Phil, Rach, Deena, Younus
• LacI - Phil, Younus, Rachel
• Swarming (2) - Harsh, Alan, Steve, Da
• Subtilin (2) - Harsh, Alan, Steve, Deena
• RocF - Harsh, Alan, Steve, Da

Wiki

Labbook

Everyone needs to carry out the following protocol for each day in the lab

1. Aim
2. Materials and Methods
3. Procedure/Protocol
4. Results
5. Conclusion

Relevant photographs should be added and labelled

General:

• Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)

UK iGEM Gathering at Newcastle - 20th/21st July

Presentation:

Introduction

Rach, Deena, Jannetta [2 mins]

• Cover Page
• Picture of us standing up
• Introduction of our iGEM project - using BS to repair concrete

General Outlook

Harsh, Da, Phil [5 mins]

• Starting with bioreactor
• Transformation into spores
• Transportation
• Spraying on building
• Germination
• sensing crack + non-environmental kill switch
• swarming
• end of crack signalling = QS
• Filamentous cells
• CaCO3

BioBrick Discussion

Alan, Younus, Steve [8 mins altogether; 2 mins each]

• Swarming
• Subtillin
• Filamentous cells - YneA
• CaCO3 - Urease
• LacI

Whats next...

[5 mins remaining] 'Save the babies!!!'

Minideadlines

1. Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
2. Cloning Strategies - to be completed by Friday, 16th July 2010.
   • YneA
   • One sequence for sfp primers and coding sequence ready (Goksel)
   • Subtilin just immunity

Action Points

1. Finish wiki updates by Sunday 18th (everyone)
2. Team page on Wiki:
   • Add Colin Harwood, Colin Daviy, Wendy Smith and Goksel Misrili to list of advisors
   • Deena, Phil and Younus to write about yourself + pictures