Team:Cambridge/Templates/boxesprototypenew

From 2010.igem.org

(Difference between revisions)

Latest revision as of 19:43, 7 June 2011

Abstract

We placed genes from fireflies and bioluminescent bacteria into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include and quantitative biosensors and biological alternatives to conventional lighting.

Multimedia

If you want a break from hard-core science, check out our Gibson Assembly music video.

You can also see view videos of our bacterial bubble lamp and project overview.

Tools

Gibson
Assembly Ligation
Calculator

@@ Line 5: / Line 5: @@
 <div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
 background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
-<p style="line-height:120%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">We placed genes from <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">prokaryotes</a> into E.coli to create the brightest bacterial light in the registry.  We  pioneered and documented the recently described <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Gibson/Introduction">Gibson Assembly</a> technique. We used site-directed mutagenesis to create a wide range of coloured bioluminescent BioBricks.</p></div>
+<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> We placed genes from <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">bacteria</a> into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include  and quantitative <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure">biosensors</a> and biological <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/Lighting">alternatives</a> to conventional lighting.</p>
+</div>
 </div>
@@ Line 13: / Line 14: @@
 <div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
 background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
-<p style="line-height:120%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">If you want a break from hard-core science, check out our Gibson Assembly  <a  style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/WCWjJFU1be8?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">music video</a>.<br /><br />You can also see a video which shows off our <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/tUFscEVK5Ks?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">bacterial bubble lamp</a>.
+<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">If you want a break from hard-core science, check out our Gibson Assembly  <a  style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/WCWjJFU1be8?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">music video</a>.<br /><br />You can also see view videos of our <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/tUFscEVK5Ks?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">bacterial bubble lamp</a> and <a style="color:#d9b400 !important" onclick="displayGibson('http://www.cambridgeigem.org/video.php'); return false;" href="http://www.cambridgeigem.org/video.php?nojs=true">project overview</a>.
 <script>
+var dontclose=false;
 function displayGibson(youTubeURL){
 var newDiv = document.createElement('div');
 newDiv.setAttribute('id',"GibsonVidOverlay");
-newDiv.setAttribute('style',"width:100%;height:100%;background:black; position:fixed; left:0; top:0;z-index:100; background:url(https://static.igem.org/mediawiki/2010/e/e9/Opacity9.png) ;");
+newDiv.setAttribute('style',"width:100%;height:100%;background:black; position:fixed; left:0; top:0;z-index:100;  text-align:center; background:url(https://static.igem.org/mediawiki/2010/e/e9/Opacity9.png) ;");
-newDiv.setAttribute('onclick',";$('body')[0].removeChild($('#GibsonVidOverlay')[0]);");
+newDiv.setAttribute('onclick',";hideOverlay();");
-newDiv.innerHTML='<div align="center" style="margin-top:100px;"><object width="640" height="385"><param name="movie" value="'+youTubeURL+'"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="'+youTubeURL+'" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="640" height="385"></embed></object></div>';
+newDiv.innerHTML='<div style="margin-top:100px; z-index:1000;padding:10px; background:#080808;width:640px; margin-left: auto; margin-right:auto;" onclick="dontclose=true;"><object width="640" height="385"><param name="movie" value="'+youTubeURL+'"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="'+youTubeURL+'" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="640" height="385"></embed></object></div><a href="#" style="color:white !important; margin-top:20px; font-size:15px;" onclick="hideOverlay()">close</a>';
 $('body')[0].appendChild(newDiv);
+}
+function hideOverlay(){
+if(dontclose){
+dontclose=false;
+}
+else{
+$('body')[0].removeChild($('#GibsonVidOverlay')[0]);
+}
 }
 </script>
@@ Line 29: / Line 39: @@
 <div id="rightbox" style="float:left;  height:200px; margin-left:5px; width:242px;" class="tophome">
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+<div id="rtop" style="background:#fb5c2b;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Tools</div>
 <div id="rcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
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-<script src="http://widgets.twimg.com/j/2/widget.js"></script>
+<div style="position:relative; top:10px;">
-<style>
+<a href="http://www.gibthon.org" id="leftbox" style="background:url('https://static.igem.org/mediawiki/2010/5/53/Cambridge-Gibthon.png'); position:absolute; width:120px; height:100px; left:0;top:0; background-repeat:no-repeat; display:block;text-align:center; padding-top:70px; background-position: center top; color:#e53500 !important;" >
-.twtr-hd{display:none;}
+Gibson<br />Assembly
-.twtr-ft{display:none;}
+</a>
-.twtr-tweet-text p{text-align:left; font-size:90%}
+<a href="http://www.gibthon.org/ligate.html" id="rightbox" style="background:url('https://static.igem.org/mediawiki/2010/b/b1/Cambridge-Flask.png'); position:absolute; width:120px; height:100px; left:120px; text-align:center;top:0; background-repeat:no-repeat;display:block; padding-top:70px; background-position: center top; color:#e53500 !important;" />
-.twtr-tweet{border-bottom:0;}
+Ligation<br />Calculator
-</style>
+</a>
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+</div>
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