Team:HokkaidoU Japan/Safety

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[[Team:HokkaidoU_Japan/Safety|English]]
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[[Team:HokkaidoU_Japan/Safety_jp|日本語]]
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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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== Safety ==
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&nbsp;&nbsp;&nbsp;The strains of E. coli that we have generated in the course of this project are not likely to cause disease or other bio-safety problems under standard laboratory conditions. None of the parts that we have generated in our project have a high inherent bio-safety risk, though it is conceivable that these parts could be used to along proteins that themselves have bio-safety issues. Therefore we stress that users of this technology verify that human risks from expressed proteins are minimal.
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&nbsp;&nbsp;&nbsp;We refered https://2010.igem.org/Team:Washington/Safety for this statement.
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== Secretion System ==
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&nbsp;&nbsp;&nbsp;Our work on Type 3 Secretion System was done without involving live Salmonella bacteria. We used 2 BAC library fragments from Salmonella's DNA which was obtained from Salmonella Genetic Stock Centre (SGSC) following required procedures. These 2 fragments of salmonella genome is inserted in BAC Vector which is contained in E.Coli strain MG1655. Our instructor obtained appropriate permit from our universities safety officer of genetic recombinantion.  These E.Coli were acknowledged as non-pathogenic and permitted to be used under P1 safety level. However due to material transfer agreement between our laboratory and SGSC we can’t submit  Type 3 Secretion Apparatus (Syringe part of Type 3 Secretion System) to BioBrick Registry.
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:HokkaidoU_Japan|Home]]
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!align="center"|[[Team:HokkaidoU_Japan/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=HokkaidoU_Japan Official Team Profile]
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!align="center"|[[Team:HokkaidoU_Japan/Project|Project]]
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!align="center"|[[Team:HokkaidoU_Japan/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:HokkaidoU_Japan/Modeling|Modeling]]
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!align="center"|[[Team:HokkaidoU_Japan/Notebook|Notebook]]
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!align="center"|[[Team:HokkaidoU_Japan/Safety|Safety]]
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==Safety==
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Please use this page to answer the safety questions posed on the [[Safety | safety page]].
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Latest revision as of 08:48, 21 April 2011

English / 日本語

Safety

   The strains of E. coli that we have generated in the course of this project are not likely to cause disease or other bio-safety problems under standard laboratory conditions. None of the parts that we have generated in our project have a high inherent bio-safety risk, though it is conceivable that these parts could be used to along proteins that themselves have bio-safety issues. Therefore we stress that users of this technology verify that human risks from expressed proteins are minimal.

   We refered https://2010.igem.org/Team:Washington/Safety for this statement.

Secretion System

   Our work on Type 3 Secretion System was done without involving live Salmonella bacteria. We used 2 BAC library fragments from Salmonella's DNA which was obtained from Salmonella Genetic Stock Centre (SGSC) following required procedures. These 2 fragments of salmonella genome is inserted in BAC Vector which is contained in E.Coli strain MG1655. Our instructor obtained appropriate permit from our universities safety officer of genetic recombinantion. These E.Coli were acknowledged as non-pathogenic and permitted to be used under P1 safety level. However due to material transfer agreement between our laboratory and SGSC we can’t submit Type 3 Secretion Apparatus (Syringe part of Type 3 Secretion System) to BioBrick Registry.

Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Safety"