Team:Cornell

From 2010.igem.org

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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{|align="justify"
{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:Cornell_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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{| style="border:1px solid #cef2e0; background:#f5fffa; color:#008811; vertical-align:top;" cellpadding="3" cellspacing="1" width="900px" align="center"
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|[[Image:Cornell_team.png|right|frame|Your team picture]]
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!align="center"|[[Team:Cornell|The Project]]
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!align="center"|[[Team:Cornell/Project/Background|Background]]
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!align="center"|[[Team:Cornell/Project/Design|Design]]
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|align="center"|[[Team:Cornell | Team Example]]
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Cornell|Home]]
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!align="center"|[[Team:Cornell/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Cornell Official Team Profile]
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!align="center"|[[Team:Cornell/Project|Project]]
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!align="center"|[[Team:Cornell/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:Cornell/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Cornell/Modeling|Modeling]]
 
!align="center"|[[Team:Cornell/Notebook|Notebook]]
!align="center"|[[Team:Cornell/Notebook|Notebook]]
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!align="center"|[[Team:Cornell/Team|The Team]]
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!align="center"|[[Team:Cornell/Outreach & Human Practices|Outreach & Human Practices]]
!align="center"|[[Team:Cornell/Safety|Safety]]
!align="center"|[[Team:Cornell/Safety|Safety]]
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!align="center"|[[Team:Cornell/Application|Application]]
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=OMG OMVs!=
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[[Image:CUGEMZoo.jpg|right|500px]]
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Outer membrane vesicles (OMVs) are natural secretions by gram-negative bacteria that
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can transport various proteins, lipids, and nucleic acids in interactions with mammalian
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host cells. OMV technology presents an affordable, non-toxic, and direct method of drug
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delivery and antigen tracking. We have designed a method for visualizing the interactions
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of mammalian cells with outer membrane vesicles by utilizing the ClyA surface protein
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as an attachment site for fluorescent proteins. The current goal of this project is to
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characterize the distribution of varying ClyA-fluorescent protein complexes on OMVs.
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Future work will be to develop a tracking system employing two ClyA constructs:  a ClyA-fluorescent protein
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fusion for in vitro microscope imaging and a ClyA-single-chain antibody fragment to bind to an antigen.

Latest revision as of 20:57, 13 January 2011

CUGEM Banner.png
The Project Background Design Parts Submitted to the Registry Notebook The Team Outreach & Human Practices Safety Application


OMG OMVs!

CUGEMZoo.jpg

Outer membrane vesicles (OMVs) are natural secretions by gram-negative bacteria that can transport various proteins, lipids, and nucleic acids in interactions with mammalian host cells. OMV technology presents an affordable, non-toxic, and direct method of drug delivery and antigen tracking. We have designed a method for visualizing the interactions of mammalian cells with outer membrane vesicles by utilizing the ClyA surface protein as an attachment site for fluorescent proteins. The current goal of this project is to characterize the distribution of varying ClyA-fluorescent protein complexes on OMVs. Future work will be to develop a tracking system employing two ClyA constructs: a ClyA-fluorescent protein fusion for in vitro microscope imaging and a ClyA-single-chain antibody fragment to bind to an antigen.

Retrieved from "http://2010.igem.org/Team:Cornell"