Team:Cambridge

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{{:Team:Cambridge/Templates/headerbar|colour=#386abc|title=The Project}}
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<div style="position:absolute; width:230px; left:5px; text-align:center;">A flask lit by E. coli transformed with one of our constructs </div>
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<div style="position:absolute; width:230px; left:240px; text-align:center;">E. coli transformed with our different coloured bioluminescent systems</div>
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Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''biosensors'''] and as [https://2010.igem.org/Team:Cambridge/Tools/Lighting '''natural light sources''']. We also developed software tools to aid construction of BioBrick parts and devices.
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We adopted a number of strategies to extend the use of '''firefly luciferase''':
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* [https://2010.igem.org/Team:Cambridge/Codons '''Codon optimisation'''] for increased light output
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* Use of a [https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration '''luciferin regenerating enzyme'''].
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* Mutagenesis to create a number of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour '''different colours''']
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We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from '''Vibrio fischeri'''.  We believe we have created the [https://2010.igem.org/Team:Cambridge/Bioluminescence/G28 '''first BioBrick'''] to emit light in normal E. coli strains without the addition of any external substrate.
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We are the  Cambridge Team for iGEM 2010, we are investigating the phenomenon  of <strong>bioluminescence</strong> in fireflies (<em>Photinus pyralis</em>), <em>Vibrio fischeri</em>, <em>Vibrio harveyi</em> and aiming to transfer one or more of these systems to biobricks.
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The aim will be to firstly provide a quantifiable photon output, and secondly to build the foundations for bioluminescent light sources in the future. In addition, we are looking into quiescence as a method to increase output.
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During our project we made extensive use of [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''] to manufacture our parts, and have submitted an [https://2010.igem.org/Team:Cambridge/Gibson/RFC '''RFC'''] to the [http://bbf.openwetware.org/ '''BioBricks Foundation'''] to help future teams make best use of this technique.
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Please read our more comprehensive [[Team:Cambridge/Description | project description]] for further details.
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Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:
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* [https://2010.igem.org/Team:Cambridge/Tools/Gibson '''Gibthon Construct Designer'''] allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''].
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* [https://2010.igem.org/Team:Cambridge/Tools/GenBank '''BioBrick → GenBank'''] allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
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* The [https://2010.igem.org/Team:Cambridge/Tools/Ligate '''Ligation Calculator'''] is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
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* The [https://2010.igem.org/Team:Cambridge/Tools/Eglometer '''E.glometer'''] is a cheap, easily built, piece of electronics for measuring bioluminescence.  It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''quantitative biosensors'''].
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This page will be updated over the course of the summer as we progress through our project.  If you would like to get in touch with us for any reason, please do [mailto:info@cambridgeigem.org email us].
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{{:Team:Cambridge/Templates/Nolineheader2|header=Achievements in iGEM competition
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* Finalist
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* Winner of "Best Wiki" award (awarded jointly to Cambridge and [https://2010.igem.org/Team:Imperial_College_London '''Imperial College London'''])
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* Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
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* Awarded a Gold Medal
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Please see [http://https://igem.org/Results '''iGEM official results page'''] to see how all the teams did.
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{{:Team:Cambridge/Templates/Nolineheader2|header=In the news
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* [http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html New Scientist article]
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* [http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html Daily Mail article]
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* [http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html The Telegraph Article]
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[[Image:Cambridge_team_pic2010.jpg|center|frame|The team - in order - Theo Sanderson, Peter Emmrich, Ben Reeve, Anja Hohmann, Emily Knott, Bill Collins, Paul Masset, Hannah Copley, Will Handley]]
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[[Image:Cambridge_team_pictwo2010.jpg|center|frame|The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins]]
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Latest revision as of 13:17, 26 December 2010

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