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| | {{ETHZ_Basel10}} | | {{ETHZ_Basel10}} |
| | + | {{ETHZ_Basel10_Introduction}} |
| | + | = E. lemming - a remotely controlled living robot by ETH Zurich = |
| | + | {{ETHZ_Basel10_Teaser}} |
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| - | == E. lemming == | + | == Sponsors == |
| - | [[Image:Setup.jpg|thumb|400px|'''Figure 1.''' Setup to control ''E. coli'' movements. An automatized microscope images the E. lemming. A connected computer system detects and tracks the cells. The direction of movement of the E. lemming is compared to the desired direction defined by the user, e.g. with a joystick. If the direction of movement deviates too much from the desired direction, the digital controller induces tumbling by sending a red light pulse. Otherwise, tumbling is repressed by sending a far-red light pulse.]]
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| - | | + | |<html><img src="https://static.igem.org/mediawiki/2010/6/65/ETHZ_Basel_sponsors.jpg"></html> |
| - | The core idea of our project is to control the movement (chemotaxis) of ''E. coli''-
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| - | by means of light! In fact, we will alter the chemotactic pathway
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| - | either by substituting the receptor with a light-sensitive one or by
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| - | interference with the kinase-phosphatase process. We will realize this with the help of proteins whose
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| - | association and dissociation can be stimulated by pulses of light waves. In both
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| - | ways, ''E. coli'' tumbling is induced or supressed simply by pressing a light
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| - | switch! As a consequence, the bacterium can be "driven" to a
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| - | precise, pre-fixed location. Tumbling / directed flagellar movement rates are monitored by a digital controller, which is combined with image processing algorithms. This system enables control of single E. coli cells: We'll make them move like mindless "Lemmings" in the direction they are forced to go!
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| - | This years ETHZ Basel project goal is to control E.coli chemotaxis by hijacking and perturbing the tumbling / directed flagellar movement apparatus. By coupling directed flagellar movement regulating proteins to a light-sensitive spatial localization system, their activity can be controlled reversibly. A light-sensitive dimerizing complex fused to this regulating proteins and a spatial fixed location is induced by light pulses and therefore localization of the two molecules can be manipulated. Tumbling / directed flagellar movement rates are supervised by image processing algorithms, which are linked to the light-pulse generator. This system enables to control single E. coli cells to move like mindless "Lemmings" in the direction they are forced to go.
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