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| - | Project abstract
| + | {{ETHZ_Basel10}} |
| | + | {{ETHZ_Basel10_Introduction}} |
| | + | = E. lemming - a remotely controlled living robot by ETH Zurich = |
| | + | {{ETHZ_Basel10_Teaser}} |
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| - | ----
| + | == Sponsors == |
| - | | + | {| border="0" align="center" |
| - | '''E. lemming'''
| + | |<html><img src="https://static.igem.org/mediawiki/2010/6/65/ETHZ_Basel_sponsors.jpg"></html> |
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| - | ETHZ Basel project goal is to control E. coli movements (chemotaxis)
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| - | by means of light. In fact, we will change the chemotaxis pathway
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| - | either by substituting the receptor with a light-sensitive one or by
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| - | interfering with the kinase-phosphatase process with proteins whose
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| - | binding and unbinding can be stimulated by pulses of light. In both
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| - | ways, E. coli tumbling is induced or removed just by pressing a light
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| - | switch and, as a consequence, a bacterium can be "driven" to a
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| - | precise, pre-fixed point. Tumbling / directed flagellar movement rates are supervised by image processing algorithms, which are linked to the light-pulse generator. This system enables to control single E. coli cells to move like mindless "Lemmings" in the direction they are forced to go.
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| - | [[Image:Setup.jpg|frame|'''Figure 1.''' Setup to control E. Coli movements. An automatized microscope images the E. lemming. A connected computer system detects and tracks the cells. The direction of movement of the E. lemming is compared to the desired direction defined by the user, e.g. with a joystick. If the direction of movement deviates too much from the desired direction, the digital controller induces tumbling by sending a red light pulse. Otherwise, tumbling is repressed by sending a far-red light pulse.]]
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| - | <!--- Old version
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| - | This years ETHZ Basel project goal is to control E.coli chemotaxis by hijacking and perturbing the tumbling / directed flagellar movement apparatus. By coupling directed flagellar movement regulating proteins to a light-sensitive spatial localization system, their activity can be controlled reversibly. A light-sensitive dimerizing complex fused to this regulating proteins and a spatial fixed location is induced by light pulses and therefore localization of the two molecules can be manipulated. --->
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| - | <!--- The Mission, Experiments --->
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| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | + | |
| - | !align="center"|[[Team:ETHZ_Basel|Home]]
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| - | !align="center"|[[Team:ETHZ_Basel/Team|Team]]
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| - | !align="center"|[https://igem.org/Team.cgi?year=2010&team_name=ETHZ_Basel Official Team Profile]
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| - | !align="center"|[[Team:ETHZ_Basel/Project|Project]]
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| - | !align="center"|[[Team:ETHZ_Basel/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:ETHZ_Basel/Modeling|Modeling]]
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| - | !align="center"|[[Team:ETHZ_Basel/Notebook|Notebook]]
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| - | !align="center"|[[Team:ETHZ_Basel/Safety|Safety]]
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| | |} | | |} |
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