Team:UCSF/Parts

From 2010.igem.org

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<h3  style="font-weight:bold;">Parts</h3>
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<h3  style="font-weight:bold;">Parts submitted to the Registry</h3>
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<p>During the summer we built the following devices under the BioBrick standard <a href="https://2009.igem.org/Judging/Variance/UCSF">RFC28</a> (see more info <a href="http://dspace.mit.edu/bitstream/handle/1721.1/46721/BBFRFC28.pdf">here</a>), which was developed by the UCSF team in 2009 and was approved by the Registry of Parts.  However, this year the Registry does not accept RFC28.  Due to time constrains, we could not move all of these parts into pSB1C3 and submit them as we had planned.</p>
+
<p>During the summer we built more than 60 devices under the BioBrick standard <a href="https://2009.igem.org/Judging/Variance/UCSF" target="_blank">RFC28</a> (see more info <a href="http://dspace.mit.edu/bitstream/handle/1721.1/46721/BBFRFC28.pdf" target="_blank">here</a>), which was developed by the UCSF team in 2009 and was approved by the Registry of Parts.  However, this year the Registry does not accept RFC28.  Due to time constrains, we could not move all of these parts into pSB1C3 and submit them as we had planned.</p><br>
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<!--
<table id="customers">
<table id="customers">
  <tr>
  <tr>
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   <td>Designed by</td>
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   <th>Designed by</th>
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   <td>Ligated parts</td>
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   <th>Ligated parts</th>
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   <td>Function</td>
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   <th>Function</th>
  </tr>
  </tr>
  <tr>
  <tr>
   <td>Carmen</td>
   <td>Carmen</td>
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   <td>Old antiMesoCAR-LFA1-Dap10</td>
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   <td>device</td>
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   <td>Stronger Signaling</td>
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   <td>purpose</td>
  </tr>
  </tr>
  <tr class="alt">
  <tr class="alt">
   <td>John</td>
   <td>John</td>
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   <td>antiCD19CAR-DAP10-STOP</td>
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   <td>device</td>
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   <td>Positive Control</td>
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   <td>purpose</td>
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</tr>
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<tr>
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  <td>Hannah</td>
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  <td>antiCD19CAR-mDAP10y85F-STOP</td>
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  <td>Stronger Signaling</td>
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  </tr>
  </tr>
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 +
</table>
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<tr class="alt">
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-->
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  <td>Lianna</td>
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  <td>antiCD19CAR-mDAP10N87Q-STOP</td>
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  <td>Negative Control</td>
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</tr>
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<tr>
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  <td>Sam</td>
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  <td>antiMESOCAR-mDAP10N87Q-STOP</td>
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  <td>Logic Gate Signaling</td>
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</tr>
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<tr class="alt">
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  <td>Crystal</td>
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  <td>antiMESOCAR-DAP10-CD3Z</td>
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  <td>Stronger Signaling</td>
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</tr>
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<tr>
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  <td>Crystal</td>
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  <td>antiMESOCAR- LFA1-CD3z</td>
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  <td>Stronger Signaling</td>
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</tr>
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+
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<tr class="alt">
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  <td>Crystal</td>
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  <td>antiMESOCAR-DAP10-DAP10</td>
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  <td>Stronger Signaling</td>
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</tr>
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<tr>
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  <td>Sam</td>
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  <td>antiCD19CAR-DAP10-CD3z</td>
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  <td>Stronger Signaling</td>
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</tr>
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+
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<tr class="alt">
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-
  <td>Sam</td>
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  <td>antiCD19CAR-DAP10-Dap10</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
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-
<tr>
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-
  <td>Sam</td>
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-
  <td>antiCD19CAR-CD3z-CD3z</td>
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-
  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
+
-
  <td>Carmen</td>
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  <td>antiMESOCAR-CD3Z-STOP</td>
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  <td>Positive Control</td>
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-
</tr>
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-
<tr>
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-
  <td>Carmen</td>
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-
  <td>antiCD19-CD3z-STOP</td>
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-
  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
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-
  <td>Carmen</td>
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  <td>antiMESOCAR-KIR3DL2-STOP</td>
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  <td>Inhibition</td>
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</tr>
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-
<tr>
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-
  <td>Carmen</td>
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-
  <td>antiCD19CAR-KIR3DL2-STOP</td>
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  <td>Inhibition</td>
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-
</tr>
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-
<tr>
+
-
  <td>Crystal</td>
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-
  <td>antiMESOCAR-LFA1-DAP10</td>
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  <td>Stronger Signaling</td>
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-
</tr>
+
-
<tr>
+
-
  <td>Crystal</td>
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-
  <td>antiMESOCAR-mDAP10 M88Q-STOP</td>
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  <td>Logic Gate Signaling</td>
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-
</tr>
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-
<tr>
+
-
  <td>Crystal</td>
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-
  <td>antiCD19CAR-mDAP10 M88Q-STOP</td>
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  <td>Logic Gate Signaling</td>
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</tr>
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-
<tr>
+
-
  <td>Lianna</td>
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-
  <td>antiMESOCAR-OX40-Dap10</td>
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-
  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
+
-
  <td>Lianna</td>
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-
  <td>antiMESOCAR-OX40-CD3z</td>
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-
  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
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-
  <td>Carmen</td>
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  <td>antiMESOCAR-FcRg-DAP10</td>
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  <td>Stronger Signaling</td>
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</tr>
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<tr>
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  <td>Carmen</td>
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  <td>antiMESOCAR-ICOS-DAP10</td>
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  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
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-
  <td>Carmen</td>
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-
  <td>antiCD19CAR-FcRg-DAP10</td>
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  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
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-
  <td>Carmen</td>
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  <td>antiCD19CAR-ICOS-DAP10</td>
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-
  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
+
-
  <td>Carmen</td>
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-
  <td>antiCD19CAR-LFA1-DAP10</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
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-
<tr>
+
-
  <td>Crystal</td>
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-
  <td>antiMESOCAR-CD3z-CD3z</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Lianna</td>
+
-
  <td>antiMESOCAR-mDAP10Y85F-STOP</td>
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  <td>Logic Gate Negative Control</td>
+
-
</tr>
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-
<tr>
+
-
  <td>Connor</td>
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-
  <td>antiCD19CAR-KIR3DL1-STOP</td>
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-
  <td>Inhibition</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Connor</td>
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-
  <td>antiMESOCAR-KIR3DL1-STOP</td>
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-
  <td>Inhibition</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Crystal</td>
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  <td>antiMESOCAR-DAP10-STOP</td>
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  <td>Stronger Signaling</td>
+
-
</tr>
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-
<tr>
+
-
  <td>Lianna</td>
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-
  <td>antiCD19CAR-OX40-DAP10</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Lianna</td>
+
-
  <td>antiCD19CAR-OX40-CD3z</td>
+
-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Carmen</td>
+
-
  <td>antiCD19CAR-SLAMF1-CD3z</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Carmen</td>
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-
  <td>antiCD19CAR-SLAMF1-DAP10</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Carmen</td>
+
-
  <td>antiCD19CAR-SLAMF5-DAP10</td>
+
-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>John</td>
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-
  <td>antiMESOCAR-SLAMF1-CD3z</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>John</td>
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-
  <td>antiMESOCAR-SLAMF5-DAP10</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Lianna</td>
+
-
  <td>antiCD19CAR-FcRg-STOP</td>
+
-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Lianna</td>
+
-
  <td>antiCD19CAR-ICOS-STOP</td>
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-
  <td>Stronger Signaling</td>
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-
</tr>
+
-
<tr>
+
-
  <td>Lianna</td>
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  <td>antiCD19CAR-CD28-STOP</td>
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  <td>Stronger Signaling</td>
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</tr>
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<tr>
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-
  <td>Lianna</td>
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-
  <td>antiMESOCAR-FcRg-STOP</td>
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  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
+
-
  <td>Lianna</td>
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  <td>antiMESOCAR-ICOS-STOP</td>
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  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
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-
  <td>Lianna</td>
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  <td>antiMESOCAR-CD28-STOP</td>
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  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
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-
  <td>Lianna</td>
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-
  <td>antiCD19CAR-LFA1-STOP</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Lianna</td>
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-
  <td>antiMESOCAR-LFA1-STOP</td>
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  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
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-
  <td>Crystal</td>
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  <td>antiCD19CAR-LFA1-CD3z</td>
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</tr>
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-
<tr>
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-
  <td>Crystal</td>
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  <td>antiMESOCAR-OX40-STOP</td>
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  <td>Stronger Signaling</td>
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-
</tr>
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-
<tr>
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-
  <td>Crystal</td>
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  <td>antiMESOCAR-SLAMF3-CD3z</td>
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  <td>Stronger Signaling</td>
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-
</tr>
+
-
<tr>
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  <td>Crystal</td>
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  <td>antiMESOCAR- CD3g-CD3z</td>
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  <td>Stronger Signaling</td>
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</tr>
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<tr>
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  <td>Crystal</td>
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  <td>antiMESOCAR-Ly49-STOP</td>
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  <td>Stronger Signaling</td>
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-
</tr>
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<tr>
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  <td>Crystal</td>
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  <td>antiMESOCAR-NKG2A-STOP</td>
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  <td>Negative Feedback</td>
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</tr>
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-
<tr>
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  <td>Crystal</td>
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  <td>antiCD19CAR-OX40-STOP</td>
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  <td>Stronger Signaling</td>
+
-
</tr>
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-
<tr>
+
-
  <td>Crystal</td>
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  <td>antiCD19CAR-SLAMF3-CD3z</td>
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  <td>Stronger Signaling</td>
+
-
</tr>
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-
<tr>
+
-
  <td>Crystal</td>
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-
  <td> antiCD19CAR-CD3g-CD3z</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Crystal</td>
+
-
  <td>antiCD19CAR-FcRg-CD3z</td>
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-
  <td>Stronger Signaling</td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
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  <td>GZMB-eGFP-CDMPR</td>
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  <td>Localization to Cytoxic Granules </td>
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-
</tr>
+
-
<tr>
+
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  <td>Min</td>
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  <td>GZMB-eGFP-GILT</td>
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  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMB-eGFP-LAMP</td>
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  <td>Localization to Cytoxic Granules </td>
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-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMB-eGFP-LIMP</td>
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  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMB-eGFP-Ymotif</td>
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-
  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMB-eGFP-STP</td>
+
-
  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMM-eGFP-CDMPR</td>
+
-
  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMM-eGFP-GILT</td>
+
-
  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMM-eGFP-LAMP</td>
+
-
  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMM-eGFP-LIMP</td>
+
-
  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMM-eGFP-Ymotif</td>
+
-
  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Min</td>
+
-
  <td>GZMM-eGFP-STP</td>
+
-
  <td>Localization to Cytoxic Granules </td>
+
-
</tr>
+
-
</table>
+
 +
<br>
 +
<br>
 +
<br>
<!-- content ends here -->
<!-- content ends here -->

Latest revision as of 22:29, 15 November 2010


Parts submitted to the Registry

<groupparts>iGEM010 UCSF</groupparts>

During the summer we built more than 60 devices under the BioBrick standard RFC28 (see more info here), which was developed by the UCSF team in 2009 and was approved by the Registry of Parts. However, this year the Registry does not accept RFC28. Due to time constrains, we could not move all of these parts into pSB1C3 and submit them as we had planned.