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Week of 10/18
From 2010.igem.org
(New page: [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook]) |
(→October 20-25th) |
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| + | ==October 19th== | ||
| + | |||
| + | *Minipreps: | ||
| + | -J23116+K091146 | ||
| + | -A3 | ||
| + | |||
| + | There had been previous minipreps completed; however, the two above did not turn out correctly the first time. Because of this problem, they were miniprepped once more. | ||
| + | |||
| + | *Digested: | ||
| + | -K091107+J23116 (EX) | ||
| + | -K091107+J23108 (EX) | ||
| + | -K091146+J23108 (EX) | ||
| + | -K091146+J13116 (EX) | ||
| + | -AFP (EX) | ||
| + | -TetCIR (EX) | ||
| + | -LTC+J23116 (ES) | ||
| + | -LTC+J23108 (ES) | ||
| + | |||
| + | Note: There was protein contamination in K091146+J23116 after the digest. | ||
| + | |||
| + | *Gel Order: | ||
| + | -Ladder | ||
| + | -K091107+J23116 | ||
| + | -K091107+J23108 | ||
| + | -K091146+J23108 | ||
| + | -AFP | ||
| + | -TetCIR | ||
| + | -LTC+J23116 | ||
| + | -LTC+J23108 | ||
| + | -K091146+J13116 | ||
| + | |||
| + | |||
| + | |||
| + | ==October 20-25th== | ||
| + | |||
| + | At this point we had started to wrap up our work. We did not keep accurate records in our Lab Notebook; however, this is when we began sequencing our final parts and constructs. | ||
| + | |||
| + | A final run on the Tecan microplate reader was carried out from Friday 22 to Sunday 24. | ||
| + | parts tested: | ||
| + | |||
| + | P6 promoter | ||
| + | D5 promoter | ||
| + | J23113 promoter | ||
| + | K091107 Lux promoter with J23108 expressing LuxR | ||
| + | K091107 Lux promoter with J23116 expressing LuxR | ||
| + | K091146 Lux promoter with J23108 expressing LuxR | ||
| + | K091146 Lux promoter with J23116 expressing LuxR | ||
| + | |||
| + | As our sequences came back as expected, and needed, we did final ligations and transformations. | ||
| + | |||
| + | ==October 26th== | ||
| + | |||
| + | FINAL DAY OF LAB WORK!!! | ||
| + | |||
| + | We placed the plasmids of the parts we were submitting to the registry into PCR strips and taped the first tube. These tubes were then placed into a large tube, sealed, and mailed to the Parts Registry to add. For a more comprehensive review of these submissions please see our [https://2010.igem.org/Team:Penn_State/Parts Parts Submitted to the Registry] page. | ||
| + | |||
| + | |||
[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | ||
Latest revision as of 03:56, 28 October 2010
October 19th
- Minipreps:
-J23116+K091146 -A3
There had been previous minipreps completed; however, the two above did not turn out correctly the first time. Because of this problem, they were miniprepped once more.
- Digested:
-K091107+J23116 (EX) -K091107+J23108 (EX) -K091146+J23108 (EX) -K091146+J13116 (EX) -AFP (EX) -TetCIR (EX) -LTC+J23116 (ES) -LTC+J23108 (ES)
Note: There was protein contamination in K091146+J23116 after the digest.
- Gel Order:
-Ladder -K091107+J23116 -K091107+J23108 -K091146+J23108 -AFP -TetCIR -LTC+J23116 -LTC+J23108 -K091146+J13116
October 20-25th
At this point we had started to wrap up our work. We did not keep accurate records in our Lab Notebook; however, this is when we began sequencing our final parts and constructs.
A final run on the Tecan microplate reader was carried out from Friday 22 to Sunday 24. parts tested:
P6 promoter D5 promoter J23113 promoter K091107 Lux promoter with J23108 expressing LuxR K091107 Lux promoter with J23116 expressing LuxR K091146 Lux promoter with J23108 expressing LuxR K091146 Lux promoter with J23116 expressing LuxR
As our sequences came back as expected, and needed, we did final ligations and transformations.
October 26th
FINAL DAY OF LAB WORK!!!
We placed the plasmids of the parts we were submitting to the registry into PCR strips and taped the first tube. These tubes were then placed into a large tube, sealed, and mailed to the Parts Registry to add. For a more comprehensive review of these submissions please see our Parts Submitted to the Registry page.
