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Team:UC Davis
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<td><table class="pikachu" width="675px"><tr><th><img class="marth" src="/wiki/images/b/b8/Projabs.jpg" width="675px"></th></tr> | <td><table class="pikachu" width="675px"><tr><th><img class="marth" src="/wiki/images/b/b8/Projabs.jpg" width="675px"></th></tr> | ||
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| - | <td class="kirby"><p class="header">CPOTATo: The Weapon Against Crosstalk</p><p class="indent">A second challenge we've tried to overcome that grew from our main project, the cI Lambda problem and the pH sensor project is the realization that our intermediate devices were causing slow growth phenotypes in our cells. This, and other observations, led us to conclude that we were witnessing the unintended interaction between our device and the host. This is not only bad, but it is also a critical challenge in general in synthetic biology. To attempt to remedy this issue in the future, we have designed CPOTATo (Crosstalk Predictive Organism Targeted Analysis Tool), a computational tool that attempts to predict potential cross-talk between a synthetic circuit and its host so that the engineer might know before starting a project what the likelihood of potentially disruptive interactions between the host and the device is. For more, <a href="https://2010.igem.org/Team:UC_Davis/Projects">click here!</a><p> | + | <td class="kirby"><p class="header"><b>Spatial Oscillation! It Makes Stripes!</b></p>Repeating patterns are an overwhelmingly common sight in nature, whether it comes in the form of a zebra's stripes or a centipede's repeated segmented body. And although it may seem a simple task to replicate this behavior, in reality, cells that undertake transformations into specific states to express a certain phenotype undergo very complicated biological processes. These biological processes often involve a cell to know where it is spatially (spatial awareness), and given the information it receives from the state of its environment, a cell may choose to perform one action over others. Our iGEM project involves building a device that when implanted in a cell allows the cell to choose from one of two states given a stimulus (in our case, the absence of light), in a project we have named "<a href='https://2010.igem.org/Team:UC_Davis/Projects'>Spatial Oscillation</a>".<br/><br/><p class="header"><b>CPOTATo: The Weapon Against Crosstalk</b></p><p class="indent">A second challenge we've tried to overcome that grew from our main project, the cI Lambda problem and the pH sensor project is the realization that our intermediate devices were causing slow growth phenotypes in our cells. This, and other observations, led us to conclude that we were witnessing the unintended interaction between our device and the host. This is not only bad, but it is also a critical challenge in general in synthetic biology. To attempt to remedy this issue in the future, we have designed CPOTATo (Crosstalk Predictive Organism Targeted Analysis Tool), a computational tool that attempts to predict potential cross-talk between a synthetic circuit and its host so that the engineer might know before starting a project what the likelihood of potentially disruptive interactions between the host and the device is. For more, <a href="https://2010.igem.org/Team:UC_Davis/Projects?3">click here!</a><p> |
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<td class="kirby"><p class="indent"> A good scientist always keeps a lab notebook at hand in order to keep track of what they do. This ensures that they have a written record of their data, allows others to retrace their steps, and most importantly of all, back up their research findings. Sift through our notebook pages to see how this project was built! </td></tr> | <td class="kirby"><p class="indent"> A good scientist always keeps a lab notebook at hand in order to keep track of what they do. This ensures that they have a written record of their data, allows others to retrace their steps, and most importantly of all, back up their research findings. Sift through our notebook pages to see how this project was built! </td></tr> | ||
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| - | <table><tr><td class="kirby" width="200px"><p class="header"><b>Overall Workplan</b></a><p | + | <table><tr><td class="kirby" width="200px"><p class="header"><b>Overall Workplan</b></a><p> |
| - | < | + | <center><a href="https://2010.igem.org/Team:UC_Davis/notebook/overallworkplan.html"><img src="https://static.igem.org/mediawiki/2010/0/03/Overallbutton.jpg"></a></center><br /> |
| + | Get the big picture of what we are trying to build! Learn the logistics behind our circuit and how we implemented biological concepts to create a robust system. | ||
| - | <td class="kirby" width="200px"><p class="header"><b> | + | <td class="kirby" width="200px"><p class="header"><b>Assembly Workplan</b></a><p> |
| + | <center><a href="https://2010.igem.org/Team:UC_Davis/notebook/assembly.html"><img src="https://static.igem.org/mediawiki/2010/3/32/Assemblymethod1.jpg"></a></center><br /> | ||
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| + | Our parts didn't just come together magically. Learn about how we assembled our parts piece by piece! Also, stay tuned for possible protocols that may save you hours on your experiment. | ||
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| + | <td class="kirby" width="200px"><p class="header"><b>Testing & Validation</b></a><p> | ||
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| + | <center><a href="https://2010.igem.org/Team:UC_Davis/notebook/c0051debug.html"><img src="https://static.igem.org/mediawiki/2010/d/d1/Sequencingbutton.jpg"></a></center><br /> | ||
| + | Building our parts also include testing our parts and ensuring that they work, since we cannot see what is actually going on with the naked eye. Learn how we identified problems, validated our experiments, and how we overcame various issues. </td></tr></table></td></tr> | ||
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Latest revision as of 03:39, 28 October 2010
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