Team:Panama/10 September 2010

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{{:Team:Panama/Header2}}
==='''September 10'''===
==='''September 10'''===
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Ter A2 ---> Terminator grown in medium with ampicillin.
Ter A2 ---> Terminator grown in medium with ampicillin.
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Ter K1 ---> Terminator grown in medium with kanamycin.
Ter K1 ---> Terminator grown in medium with kanamycin.
Line 10: Line 10:
Ter K2 ---> Terminator grown in medium with kanamycin.
Ter K2 ---> Terminator grown in medium with kanamycin.
 +
'''DNA concentration measurements:'''
-
DNA quantification with Qubit fluorometer (invitrogen).
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DNA quantification with Qubit fluorometer (Invitrogen).
 +
Plasmid Terminator Concentrations:
-
Samples
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24.9ug/mL T1    (A1).
-
1 uL sample + 199 uL WB.
+
19.4ug/mL T2    (A2).
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Standars
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32.5ug/mL T3    (K1).
-
10 uL standards + 190 uL WB.
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28.7ug/mL T4    (K2).
-
Measurements:
 
-
Terminator
+
'''Agarose gel 1%.'''               
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24.9 ug/mL T1    (A1).
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Digestion Reactions of Plasmid,Reporter & Terminator.
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19.4 ug/mL T2    (A2).
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'''Reporter'''
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[[Image:Cuadro10septreport.JPG|200px|thumb|left|alt text]]
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32.5 ug/mL T3    (K1).
 
-
28.7 ug/mL T4    (K2).
 
-
Agarose gel 1%.               
 
 +
'''Plasmid'''
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Digest, Terminator & Reporter reactions.
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[[Image:Cuadro10septplasmid.JPG|200px|thumb|left|alt text]]
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'''Reporter      '''
 
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Buffer 2      5    uL       
 
-
BSA          0.5  uL       
 
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EcoR1        1    uL       
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'''Terminator'''
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Spe1          1    uL       
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[[Image:Cuadro10septreport.JPG|200px|thumb|left|alt text]]
-
DNA          20  uL       
 
-
H2O          22.5 uL       
 
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Final volume  50  uL       
 
-
'''Plasmid'''
 
-
Buffer 2      5    uL       
 
-
BSA          0.5  uL     
 
-
EcoR1        1    uL     
 
-
Spe1          1    uL     
 
-
DNA          20  uL     
 
-
H2O          22.5 uL     
 
-
Final volume  50  uL     
 
-
'''Terminator'''        
+
        
-
Buffer 2      5    uL       
+
This was placed for 3 hours at 37°C on the thermocycler. It takes 20 minutes at 80°C to deactivate enzymes.
-
BSA          0.5  uL       
 
-
Xba          1    uL       
 
-
Pst1          1    uL       
 
-
DNA          20  uL       
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Rh1AB(Pseudomona) PCR amplification: Second Test
-
 
+
-
H2O          22.5 uL       
+
-
 
+
-
Final volume  50  uL       
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-
 
+
-
 
+
-
This was placed 3 hours at 37° C on the thermocycler.
+
-
 
+
-
It takes 20 minutes at 80° C to deactivate enzymes.
+
-
 
+
-
PCR DNAg. Pseudomona (Rh1AB)
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II Test. Par of primers:
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Primers:
Primers:
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34.1 nMoles X 10= 341 uL H2O
34.1 nMoles X 10= 341 uL H2O
-
Resuspending with 341 uL H2O [ ]= 100 uMolar stock.
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Resuspending with 341uL H2O [ ]= 100 uMolar stock.
Work solution (10 uMolar)
Work solution (10 uMolar)
-
10 uL (Stock 100 uMolar) + 90 uL H2O
 
-
[ ]= 10 uMolar
 
-
 
-
VC = VC
 
-
(X)(100um) = (100 uL) (10 um)
 
-
X    = 10 uL del stock (100 uMolar).
 
'''2). RhT-2b'''
'''2). RhT-2b'''
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46.6 nMoles= 9.000260
46.6 nMoles= 9.000260
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46.6 nM x 10= 466 uL H2O
+
46.6 nM x 10= 466uL H2O
-
Resuspending in 466 uL H2O [ ]= 100  uMolar stock
+
Resuspending in 466uL H2O [ ]= 100  uMolar stock
Work solution (10 uM)
Work solution (10 uM)
-
VC= VC
 
-
(X)(100 uM)=(100 uL) (10 uM)
+
We tried with 3 differents volumes
-
X= 10  uL of Stock
+
1) 2.5uL [1 um] Final volume= 25uL
-
10  uL Stock + 90  uL H2O [ ]= 10 uMolar.
+
2) 1.25uL [0.5 um] Final volume= 25uL
-
We tried with 3 differents volumes
+
3) 1uL [0.4 um] Final volume= 25uL
-
1) 2.5 uL [1 um] Final volume= 25 uL
 
-
2) 1.25 uL [0.5 um] Final volume= 25 uL
+
Thermocycler Program
-
3) 1 uL [0.4 um] Final volume= 25 uL
+
1) 94° C 5 mins.
 +
 
 +
2) 94° C 30 segs.
 +
 
 +
51° C 1 min.
 +
 
 +
72° C 3 min.
 +
 
 +
3) 72° C 10 mins.
 +
 
 +
 
 +
'''PCR Reaction 1'''
 +
 
 +
 
 +
[[Image:Untitled10sept.JPG|200px|thumb|left|alt text]]
 +
 
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 +
 
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'''PCR Reaction 2'''
 +
 
 +
[[Image:Untitled10sept2.JPG|200px|thumb|left|alt text]]
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
'''PCR Reaction 3'''
 +
 
 +
[[Image:untitled10sept3.JPG|200px|thumb|left|alt text]]
-
Program
 
-
1) 94° C 5mins
 
-
2) 94° C 30seg
 
-
51° C 1mins
 
-
72° C 3mins
 
-
3) 72° C 10mins
 
-
1) H2O 6.5 uL
 
-
Primer RhT-F1b 2.5 uL
 
-
Primer RhT-2b 2.5 uL
 
-
Master mix 12.5 uL
 
-
DNA 1 uL
 
-
Final volume= 25 uL
 
-
2) H2O 9 uL
 
-
Primer RhT-2b 1.25 uL
 
-
Primer Rht-F1b 1.25 uL
 
-
Master mix 12.5 uL
 
-
DNA 1 uL
 
-
Final volume= 25 uL
 
-
3) H2O 9.5 uL
 
-
Primer F1b 1uL
 
-
Primer 2b 1mL
 
-
Master mix 12.5 uL
 
-
DNA 1 uL
 
-
Final volume= 25 uL
 
1% Gel Terminators (Extraction)
1% Gel Terminators (Extraction)
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 +
[[Image:Cuadro10septiembre.jpg|300px|thumb|left|alt text]]
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 +
 +
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[[Image:Gel10sept.JPG|200px|thumb|left|alt text]]
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 +
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 +
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 +
-
1. Hind III
 
-
1 uL Marker
 
-
+2 uL loading
 
-
+7 uL H2O
 
-
2. Ter A1
 
-
5uL sample
 
-
+2 uL loading
 
-
3. Ter A2
 
-
5uL sample
 
-
+2 uL loading
 
-
3. Ter K2
 
-
5uL sample
 
-
+2 uL loading
+
{{:Team:Panama/calendar2}}

Latest revision as of 03:22, 28 October 2010

iGEM Panama

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September 10

Ter A1 ---> Terminator grown in medium with ampicillin.

Ter A2 ---> Terminator grown in medium with ampicillin.

Ter K1 ---> Terminator grown in medium with kanamycin.

Ter K2 ---> Terminator grown in medium with kanamycin.

DNA concentration measurements:

DNA quantification with Qubit fluorometer (Invitrogen).

Plasmid Terminator Concentrations:

24.9ug/mL T1 (A1).

19.4ug/mL T2 (A2).

32.5ug/mL T3 (K1).

28.7ug/mL T4 (K2).


Agarose gel 1%.

Digestion Reactions of Plasmid,Reporter & Terminator.


Reporter

alt text




Plasmid

alt text



Terminator

alt text









This was placed for 3 hours at 37°C on the thermocycler. It takes 20 minutes at 80°C to deactivate enzymes.



Rh1AB(Pseudomona) PCR amplification: Second Test

Primers:

RhT-F1b5´ GTT TGC CTG TTC GAA AAT T 3´

RhT-2b 5´ CGA TAC GGC AAA ATC ATG G 3´

I. Resuspending the lyophilized primers for the solution stock (100 Plasmid u molar).

1). RhT-F1b

Tm 49.9° C, 34.1 nMoles= 6.10D260.

MW 5808.8

34.1 nMoles X 10= 341 uL H2O

Resuspending with 341uL H2O [ ]= 100 uMolar stock.

Work solution (10 uMolar)


2). RhT-2b

Tm= 51.7° C

MW= 5845.9

46.6 nMoles= 9.000260

46.6 nM x 10= 466uL H2O

Resuspending in 466uL H2O [ ]= 100 uMolar stock

Work solution (10 uM)


We tried with 3 differents volumes

1) 2.5uL [1 um] Final volume= 25uL

2) 1.25uL [0.5 um] Final volume= 25uL

3) 1uL [0.4 um] Final volume= 25uL


Thermocycler Program

1) 94° C 5 mins.

2) 94° C 30 segs.

51° C 1 min.

72° C 3 min.

3) 72° C 10 mins.


PCR Reaction 1


alt text




PCR Reaction 2

alt text




PCR Reaction 3

alt text














1% Gel Terminators (Extraction)

alt text








alt text
















May
MTWTFSS
          [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_May_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_May_2010&action=edit 2]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_May_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Panama/4_May_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Panama/5_May_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Panama/6_May_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Panama/7_May_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Panama/8_May_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Panama/9_May_2010&action=edit 9]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/10_May_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Panama/11_May_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Panama/12_May_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Panama/13_May_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Panama/14_May_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Panama/15_May_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Panama/16_May_2010&action=edit 16]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_May_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Panama/18_May_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Panama/19_May_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Panama/20_May_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Panama/21_May_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Panama/22_May_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Panama/23_May_2010&action=edit 23]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/24_May_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Panama/25_May_2010&action=edit 25] [http://2010.igem.org/Team:Panama/26_May_2010 26] [http://2010.igem.org/wiki/index.php?title=Team:Panama/27_May_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Panama/28_May_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Panama/29_May_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Panama/30_May_2010&action=edit 30]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/31_May_2010&action=edit 31]
June
MTWTFSS
  [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_June_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_June_2010&action=edit 2] [http://2010.igem.org/Team:Panama/3_June_2010 3] [http://2010.igem.org/Team:Panama/4_June_2010 4] [http://2010.igem.org/wiki/index.php?title=Team:Panama/5_June_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Panama/6_June_2010&action=edit 6]
[http://2010.igem.org/Team:Panama/7_June_2010 7] [http://2010.igem.org/wiki/index.php?title=Team:Panama/8_June_2010&action=edit 8] [http://2010.igem.org/Team:Panama/9_June_2010 9] [http://2010.igem.org/wiki/index.php?title=Team:Panama/10_June_2010&action=edit 10] [http://2010.igem.org/Team:Panama/11_June_2010 11] [http://2010.igem.org/wiki/index.php?title=Team:Panama/12_June_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Panama/13_June_2010&action=edit 13]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_June_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Panama/15_June_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Panama/16_June_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Panama/17_June_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Panama/18_June_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Panama/19_June_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Panama/20_June_2010&action=edit 20]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/21_June_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Panama/22_June_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Panama/23_June_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Panama/24_June_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Panama/25_June_2010&action=edit 25] [http://2010.igem.org/Team:Panama/26_June_2010 26] [http://2010.igem.org/wiki/index.php?title=Team:Panama/27_June_2010&action=edit 27]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/28_June_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Panama/29_June_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Panama/30_June_2010&action=edit 30]
July
MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_July_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Panama/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Panama/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/5_July_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Panama/6_July_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Panama/7_July_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Panama/8_July_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Panama/9_July_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Panama/10_July_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Panama/11_July_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/12_July_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Panama/13_July_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Panama/14_July_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Panama/15_July_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Panama/16_July_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Panama/17_July_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Panama/18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/19_July_2010&action=edit 19] [http://2010.igem.org/Team:Panama/20_July_2010 20] [http://2010.igem.org/Team:Panama/21_July_2010 21] [http://2010.igem.org/Team:Panama/22_July_2010 22] [http://2010.igem.org/Team:Panama/23_July_2010 23] [http://2010.igem.org/Team:Panama/24_July_2010 24] [http://2010.igem.org/wiki/index.php?title=Team:Panama/25_July_2010&action=edit 25]
[http://2010.igem.org/Team:Panama/26_July_2010 26] [http://2010.igem.org/Team:Panama/27_July_2010 27] [http://2010.igem.org/Team:Panama/28_July_2010 28] [http://2010.igem.org/Team:Panama/29_July_2010 29] [http://2010.igem.org/Team:Panama/30_July_2010 30] [http://2010.igem.org/wiki/index.php?title=Team:Panama/31_July_2010&action=edit 31]
August
MTWTFSS
            [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_August_2010&action=edit 1]
[http://2010.igem.org/Team:Panama/2_August_2010 2] [http://2010.igem.org/wiki/index.php?title=Team:Panama/3_August_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Panama/4_August_2010&action=edit 4] [http://2010.igem.org/Team:Panama/5_August_2010 5] [http://2010.igem.org/wiki/index.php?title=Team:Panama/6_August_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Panama/7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Panama/8_August_2010&action=edit 8]
[http://2010.igem.org/Team:Panama/9_August_2010 9] [http://2010.igem.org/Team:Panama/10_August_2010 10] [http://2010.igem.org/Team:Panama/11_August_2010 11] [http://2010.igem.org/Team:Panama/12_August_2010 12] [http://2010.igem.org/Team:Panama/13_August_2010 13] [http://2010.igem.org/wiki/index.php?title=Team:Panama/14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Panama/15_August_2010&action=edit 15]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/16_August_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Panama/17_August_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Panama/18_August_2010&action=edit 18] [http://2010.igem.org/Team:Panama/19_August_2010 19] [http://2010.igem.org/Team:Panama/20_August_2010 20] [http://2010.igem.org/wiki/index.php?title=Team:Panama/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Panama/22_August_2010&action=edit 22]
[http://2010.igem.org/Team:Panama/23_August_2010 23] [http://2010.igem.org/Team:Panama/24_August_2010 24] [http://2010.igem.org/wiki/index.php?title=Team:Panama/25_August_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Panama/26_August_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Panama/27_August_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Panama/28_August_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Panama/29_August_2010&action=edit 29]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_August_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Team:Panama/31_August_2010&action=edit 31]
September
MTWTFSS
    [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_September_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_September_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Panama/3_September_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Panama/4_September_2010&action=edit 4] [http://2010.igem.org/Team:Panama/5_September_2010 5]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_September_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Panama/7_September_2010&action=edit 7] [http://2010.igem.org/Team:Panama/8_September_2010 8] [http://2010.igem.org/Team:Panama/9_September_2010 9] [http://2010.igem.org/Team:Panama/10_September_2010 10] [http://2010.igem.org/wiki/index.php?title=Team:Panama/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Panama/12_September_2010&action=edit 12]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/13_September_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Panama/14_September_2010&action=edit 14] [http://2010.igem.org/Team:Panama/15_September_2010 15] [http://2010.igem.org/Team:Panama/16_September_2010 16] [http://2010.igem.org/wiki/index.php?title=Team:Panama/17_September_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Panama/18_September_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Panama/19_September_2010&action=edit 19]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/20_September_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Panama/21_September_2010&action=edit 21] [http://2010.igem.org/Team:Panama/22_September_2010 22] [http://2010.igem.org/Team:Panama/23_September_2010 23] [http://2010.igem.org/Team:Panama/24_September_2010 24] [http://2010.igem.org/wiki/index.php?title=Team:Panama/25_September_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Panama/26_September_2010&action=edit 26]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/27_September_2010&action=edit 27] [http://2010.igem.org/Team:Panama/28_September_2010 28] [http://2010.igem.org/wiki/index.php?title=Team:Panama/29_September_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Panama/30_September_2010&action=edit 30]
October
MTWTFSS
        [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_October_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_October_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Panama/3_October_2010&action=edit 3]
[http://2010.igem.org/Team:Panama/4_October_2010 4] [http://2010.igem.org/wiki/index.php?title=Team:Panama/5_October_2010&action=edit 5] [http://2010.igem.org/Team:Panama/6_October_2010 6] [http://2010.igem.org/Team:Panama/7_October_2010 7] [http://2010.igem.org/Team:Panama/8_October_2010 8] [http://2010.igem.org/wiki/index.php?title=Team:Panama/9_October_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Panama/10_October_2010&action=edit 10]
[http://2010.igem.org/Team:Panama/11_October_2010 11] [http://2010.igem.org/Team:Panama/12_October_2010 12] [http://2010.igem.org/Team:Panama/13_October_2010 13] [http://2010.igem.org/Team:Panama/14_October_2010 14] [http://2010.igem.org/Team:Panama/15_October_2010 15] [http://2010.igem.org/Team:Panama/16_October_2010 16] [http://2010.igem.org/wiki/index.php?title=Team:Panama/17_October_2010&action=edit 17]
[http://2010.igem.org/Team:Panama/18_October_2010 18] [http://2010.igem.org/Team:Panama/19_October_2010 19] [http://2010.igem.org/Team:Panama/20_October_2010 20] [http://2010.igem.org/Team:Panama/21_October_2010 21] [http://2010.igem.org/wiki/index.php?title=Team:Panama/22_October_2010&action=edit 22] [http://2010.igem.org/Team:Panama/23_October_2010 23] [http://2010.igem.org/Team:Panama/24_October_2010 24]
[http://2010.igem.org/Team:Panama/25_October_2010 25] [http://2010.igem.org/Team:Panama/26_October_2010 26] [http://2010.igem.org/Team:Panama/27_October_2010 27] [http://2010.igem.org/wiki/index.php?title=Team:Panama/28_October_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Panama/29_October_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Panama/30_October_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Team:Panama/31_October_2010&action=edit 31]
Retrieved from "http://2010.igem.org/Team:Panama/10_September_2010"