Team:IvyTech-South Bend

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== PROJECT DESCRIPTION: ==
== PROJECT DESCRIPTION: ==
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Water quality is an important recreational and general quality of living issue in northern Indiana as elsewhere.  Public beaches can often be temporarily closed because of sewage contamination of the water.  This is a direct effect of the antiquated water works in towns along the lakeshore and towns  lining the tributaries such as the St. Joseph River that passes the Ivy Tech campus.  During times of heavy rain fall the storm sewers and the sanitary sewers discharges can mix resulting in sewage discharge into the local body of water.
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Anyone who wants to enjoy bathing in natural bodies of water in or near areas populated by humans or livestock may encounter unsafe levels of enteric bacteria and other opportunists.  Contemporary methods of assessing water quality have a slow turn-around time so we have taken steps to perfect a biosensor for rapidly indirectly quantifying the presence of enteric bacteria in natural water samples through the detection of quorum sensing factors. Previous IGEMS have exploited the LuxR/pLux system for the detection of a variety of N-acylhomoserine lactone autoinducers.  We have taken steps to further perfect a biosensor based on this device by transforming a gram-positive bacteria host to eliminate any background autoinducer signal and to build-in an enzymatic “read-out” to obtain an analog output.  We envision the development of a handheld monitor that uses this IGEM biosensor, immobilized on input paper strips, to rapidly detect unsafe levels of enteric bacteria in water samples.  
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Monitoring water quality is complicated by the fact that the test for presence and quantity of coliform bacteria requires a 2 days of incubation for results.  For swimmers and other beach enthusiasts a rapid quantitative water test would be desirable.  The Ivy Tech-South Bend team, new to IGEM, is planning to building an IGEM from registry parts.  In particular we hope to marry the “stop light” device developed by the British Columbia team in 2009 with a circuit built from registry elements that can be used to detect bacterial quorum sensing proteins.  Our device will ideally detect the presence and relative concentration of quorum sensing proteins in suspect waters thus indirectly measuring the extent of contamination and therefore safety.
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Latest revision as of 03:22, 28 October 2010


Our iGEM team is composed primarily of Ivy Tech Biotech students, most of which are getting credit for their program capstone course (BIOT 280). Our team is open to others...please inquire...gtwaddle@ivytech.edu

PROJECT DESCRIPTION:

Anyone who wants to enjoy bathing in natural bodies of water in or near areas populated by humans or livestock may encounter unsafe levels of enteric bacteria and other opportunists. Contemporary methods of assessing water quality have a slow turn-around time so we have taken steps to perfect a biosensor for rapidly indirectly quantifying the presence of enteric bacteria in natural water samples through the detection of quorum sensing factors. Previous IGEMS have exploited the LuxR/pLux system for the detection of a variety of N-acylhomoserine lactone autoinducers. We have taken steps to further perfect a biosensor based on this device by transforming a gram-positive bacteria host to eliminate any background autoinducer signal and to build-in an enzymatic “read-out” to obtain an analog output. We envision the development of a handheld monitor that uses this IGEM biosensor, immobilized on input paper strips, to rapidly detect unsafe levels of enteric bacteria in water samples.

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