Team:Georgia State

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Latest revision as of 03:21, 28 October 2010

Pichia Pastoris as a host organism for iGEM

Pichia pastoris is a methylotrophic yeast used as an alternative host for protein production in addition to Escherichia coli and Saccharomyces cerevisiae. There are several reasons why P. Pastoris is an ideal host organism. Its ability to perform eukaryotic post-translational modifications, high yields of recombinant protein, and its genetic similarity to S. cerevisiae are very attractive traits (Cereghino and Cregg, 2000).

Because of these reasons, P.pastoris has quickly gained popularity for recombinant protein production. The Georgia State 2010 team believes P. pastoris would be an excellent chassis for the iGEM competition. Our goal is to provide a tool box of parts necessary for the genetic manipulation of this organism. Parts will include a plasmid backbone, several parts providing alternative selectivity options and promoter systems In addition, our tool box will be used to produce a flu virus antigen in P. pastoris as an example of how this system could be used for vaccine production. We hope our contributions will enable future users to maximize the use and further explore the incredible potential P. pastoris has to offer!

== Check out the current toolbox here!! == Team:Georgia State/Parts

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-<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+{{Georgia_State/Header}}
-<html>
-<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
-<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
-This is a template page. READ THESE INSTRUCTIONS.
-</div>
-<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
-You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
-</div>
-<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
-You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace.
-</div>
-</div>
-</html>
-<!-- *** End of the alert box *** -->
-{|align="justify"
-|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
-|[[Image:GSUteamphoto.JPG|300px|right|frame]]
-|-
-|
-''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
-|[[Image:Georgia_State_team.png|right|frame|Your team picture]]
-We are from gsu.
-|-
-|
-|align="center"|[[Team:Georgia_State | Team Example]]
-|}
-<!--- The Mission, Experiments --->
+[[Image:GSUteamphoto.jpg|900px|center]]
-{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
-!align="center"|[[Team:Georgia_State|Home]]
+==Pichia Pastoris as a host organism for iGEM==
-!align="center"|[[Team:Georgia_State/Team|Team]]
+[[Image:Pichia_pastoris.jpg|left|330px]]
-!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Georgia_State Official Team Profile]
-!align="center"|[[Team:Georgia_State/Project|Project]]
+''Pichia pastoris'' is a methylotrophic yeast used as an alternative host for protein production in addition to ''Escherichia coli'' and ''Saccharomyces cerevisiae''. There are several reasons why ''P. Pastoris'' is an ideal host organism. Its ability to perform eukaryotic post-translational modifications, high yields of recombinant protein, and its genetic similarity to ''S. cerevisiae'' are very attractive traits (''Cereghino and Cregg'', 2000).
-!align="center"|[[Team:Georgia_State/Parts|Parts Submitted to the Registry]]
-!align="center"|[[Team:Georgia_State/Modeling|Modeling]]
-!align="center"|[[Team:Georgia_State/Notebook|Notebook]]
+[[Image:Pichia_tool_box_picture.jpg|right]]
-!align="center"|[[Team:Georgia_State/Safety|Safety]]
-|}
+Because of these reasons, ''P.pastoris'' has quickly gained popularity for recombinant protein production. The Georgia State 2010 team believes ''P. pastoris'' would be an excellent chassis for the iGEM competition. Our goal is to provide a tool box of parts necessary for the genetic manipulation of this organism. Parts will include a plasmid backbone, several parts providing alternative selectivity options and promoter systems In addition, our tool box will be used to produce a flu virus antigen in ''P. pastoris'' as an example of how this system could be used for vaccine production. We hope our contributions will enable future users to maximize the use and further explore the incredible potential ''P. pastoris'' has to offer!
+== '''Check out the current toolbox here!!''' == [[Team:Georgia State/Parts]]
+{{Georgia_State/Footer}}