Team:Heidelberg/Notebook/Capsid Shuffling/ViroBytes

From 2010.igem.org

(Difference between revisions)
(→ViroBytes)
 
(12 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Heidelberg/Template}}
+
{{:Team:Heidelberg/Single}}
-
{{:Team:Heidelberg/Pagetop|note_virobytes}}
+
{{:Team:Heidelberg/Single_Pagetop|note_virobytes}}
 +
{{:Team:Heidelberg/Side_Top}}
-
{{:Team:Heidelberg/Pagemiddle}}
+
{| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#78b41e; border: 1.5px solid #333333;"
-
{| cellpadding="5" cellspacing="0" style="text-align: center; color:#009be1; border: 3px solid #009be1;"
+
|- border="0"
|- border="0"
-
! colspan="7" style="background:#009be1;" | [https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/August<font color="white">August</font>]
+
! colspan="7" style="background:#009be1;" | [https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September<font color="#ffecba">September</font>]
|- style="background:#009be1; color:white"
|- style="background:#009be1; color:white"
|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
-
|-  
+
|- style="background:#f2f2f2; color:#78b41e"
-
|colspan="6"| ||'''1'''
+
|colspan="2"| ||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#01.2F09.2F2010 1]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#02.2F09.2F2010 2]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#03.2F09.2F2010 3]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#04.2F09.2F2010 4]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#05.2F09.2F2010 5]'''
-
|-
+
|- style="background:#f2f2f2; color:#78b41e"
-
|'''2'''||'''3'''||'''4'''||'''5'''||'''6'''||'''7'''||'''8'''
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#06.2F09.2F2010 6]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#07.2F09.2F2010 7]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#08.2F09.2F2010 8]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#09.2F09.2F2010 9]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#10.2F09.2F2010 10]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#11.2F09.2F2010 11]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#12.2F09.2F2010 12]'''
-
|-
+
|- style="background:#f2f2f2; color:#78b41e"
-
|'''9'''||'''10'''||'''11'''||'''12'''||'''13'''||'''14'''||'''15'''
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#13.2F09.2F2010 13]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#14.2F09.2F2010 14]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#15.2F09.2F2010 15]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#16.2F09.2F2010 16]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#17.2F09.2F2010 17]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#18.2F09.2F2010 18]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#19.2F09.2F2010 19]'''
-
|-
+
|- style="background:#f2f2f2; color:#78b41e"
-
|'''16'''||'''17'''||'''18'''||'''19'''||'''20'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#21/08/2010|21]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#22/08/2010|22]]'''
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#20.2F09.2F2010 20]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#21.2F09.2F2010 21]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#22.2F09.2F2010 22]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#23.2F09.2F2010 23]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#24.2F09.2F2010 24]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#25.2F09.2F2010 25]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#26.2F09.2F2010 26]'''
-
|-
+
|- style="background:#f2f2f2; color:#78b41e"
-
|'''[[Igem2010/Main/synthetic_miR_Kit/August#23/08/2010|23]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#21/08/2010|24]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#21/08/2010|25]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#26/08/2010|26]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#27/08/2010|27]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#28/08/2010|28]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#29/08/2010|29]]'''
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#27.2F09.2F2010 27]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#28.2F09.2F2010 28]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#29.2F09.2F2010 29]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September#22.2F30.2F2010 30]'''||colspan="3"|
-
|-
+
|- style="background:#f2f2f2; color:#78b41e"
-
|'''[[Igem2010/Main/synthetic_miR_Kit/August#30/08/2010|30]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/August#31/08/2010|31]]'''||colspan="5"|
+
-
|}
+
-
 
+
-
{| cellpadding="5" cellspacing="0" style="text-align: center; color:#f3d675; border: 3px solid #4e93a4;"
+
-
|- border="0"
+
-
! colspan="7" style="background:#4e93a4;" | [https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/September<font color="#ffecba">September</font>]
+
-
|- style="background:#4e93a4; color:white"
+
-
|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
+
-
|-
+
-
|colspan="2"| ||'''[[Igem2010/Main/synthetic_miR_Kit/September#01/09/2010|1]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#02/09/2010|2]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#03/09/2010|3]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#04/09/2010|4]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#05/09/2010|5]]'''
+
-
|-
+
-
|'''[[Igem2010/Main/synthetic_miR_Kit/September#06/09/2010|6]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#07/09/2010|7]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#08/09/2010|8]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#09/09/2010|9]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#10/09/2010|10]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#11/09/2010|11]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#12/09/2010|12]]'''
+
-
|-
+
-
|'''[[Igem2010/Main/synthetic_miR_Kit/September#13/09/2010|13]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#14/09/2010|14]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#15/09/2010|15]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#16/09/2010|16]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#17/09/2010|17]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#18/09/2010|18]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#19/09/2010|19]]'''
+
-
|-
+
-
|'''[[Igem2010/Main/synthetic_miR_Kit/September#20/09/2010|20]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#21/09/2010|21]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#22/09/2010|22]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#23/09/2010|23]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#24/09/2010|24]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#25/09/2010|25]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#26/09/2010|26]]'''
+
-
|-
+
-
|'''[[Igem2010/Main/synthetic_miR_Kit/September#27/09/2010|27]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#28/09/2010|28]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#29/09/2010|29]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/September#30/09/2010|30]]'''||colspan="3"|
+
-
|-  
+
| colspan="7"| <span style="color:#ffffff">-</span>
| colspan="7"| <span style="color:#ffffff">-</span>
|}
|}
-
{| cellpadding="5" cellspacing="0" style="text-align: center; color:#f3d675; border: 3px solid #4e93a4;"
+
 
 +
{| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#f09600; border: 1.5px solid #333333;"
|- border="0"
|- border="0"
-
! colspan="7" style="background:#4e93a4;" | [https://2010.igem.org/Igem2010/Main/ViroBytes/October<font color="#ffecba">October</font>]
+
! colspan="7" style="background:#78b41e;" | [https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October<font color="white">October</font>]
-
|- style="background:#4e93a4; color:white"
+
|- style="background:#78b41e; color:white"
|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
-
|-  
+
|- style="background:#f2f2f2; color:#78b41e"
-
|colspan="4"| ||'''[[Igem2010/Main/synthetic_miR_Kit/October#01/10/2010|01]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#02/10/2010|02]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#03/10/2010|03]]'''
+
|colspan="4"| ||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#01.2F10.2F2010 1]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#02.2F10.2F2010 2]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#03.2F10.2F2010 3]'''
-
|-
+
|- style="background:#f2f2f2; color:#78b41e"
-
|'''[[Igem2010/Main/synthetic_miR_Kit/October#04/10/2010|04]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#05/10/2010|05]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#06/10/2010|06]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#07/10/2010|07]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#08/10/2010|08]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#09/10/2010|09]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#10/10/2010|10]]'''
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#04.2F10.2F2010 4]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#05.2F10.2F2010 5]'''
-
|-
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#06.2F10.2F2010 6]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#07.2F10.2F2010 7]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#08.2F10.2F2010 8]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#09.2F10.2F2010 9]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#10.2F10.2F2010 10]'''
-
|'''[[Igem2010/Main/synthetic_miR_Kit/October#11/10/2010|11]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#12/10/2010|12]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#13/10/2010|13]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#14/10/2010|14]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#15/10/2010|15]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#16/10/2010|16]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#17/10/2010|17]]'''
+
|- style="background:#f2f2f2; color:#78b41e"
-
|-
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#11.2F10.2F2010 11]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#12.2F10.2F2010 12]'''|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#13.2F10.2F2010 13]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#14.2F10.2F2010 14]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#15.2F10.2F2010 15]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#16.2F10.2F2010 16]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#17.2F10.2F2010 17]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#18.2F10.2F2010 18]'''
-
|'''[[Igem2010/Main/synthetic_miR_Kit/October#18/10/2010|18]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#19/10/2010|19]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#20/10/2010|20]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#21/10/2010|21]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#22/10/2010|22]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#23/10/2010|23]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#24/10/2010|24]]'''
+
|- style="background:#f2f2f2; color:#78b41e"
-
|-
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#19.2F10.2F2010 19]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#20.2F10.2F2010 20]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#21.2F10.2F2010 21]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#22.2F10.2F2010 22]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#23.2F10.2F2010 23]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#24.2F10.2F2010 24]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#25.2F10.2F2010 25]'''
-
|'''[[Igem2010/Main/synthetic_miR_Kit/October#25/10/2010|25]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#26/10/2010|26]]'''||'''[[Igem2010/Main/synthetic_miR_Kit/October#27/10/2010|27]]'''||'''28'''||'''29'''||'''30'''||'''31'''
+
|- style="background:#f2f2f2; color:#78b41e"
-
|-  
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#26.2F10.2F2010 26]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#27.2F10.2F2010 27]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#28.2F10.2F2010 28]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#29.2F10.2F2010 29]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/ViroBytes/October#22.2F30.2F2010 30]'''||colspan="3"|
-
| colspan="7"| <span style="color:#ffffff">-</span>
+
|- style="background:#f2f2f2; color:#78b41e"
 +
|colspan="7"|
 +
<span style="color:#ffffff">-</span>
|}
|}
-
{{:Team:Heidelberg/Bottom}}
+
{{:Team:Heidelberg/Side_Bottom}}
 +
 
 +
__NOTOC__
 +
=ViroBytes=
 +
 
 +
Virobytes is a rational approach to fragment-based DNA shuffling of Adeno-associated viral Cap genes. The method is complementary to our conventional [https://2010.igem.org/Team:Heidelberg/Project/Capsid_Shuffling/Homology_Based homology based DNA shuffling]. ViroByte strategy is based on [https://2009.igem.org/Team:Alberta BioByte] protocol which have been modified in order to enable for randomized fragment assembly.
 +
 
 +
<br />
 +
 
 +
[[Image:Virobytes_shuff.jpg|thumb|350px|left|ViroBytes Assembly Scheme]]
 +
 
 +
{{:Team:Heidelberg/Single_Bottom}}

Latest revision as of 03:20, 28 October 2010


ViroBytes

Virobytes is a rational approach to fragment-based DNA shuffling of Adeno-associated viral Cap genes. The method is complementary to our conventional homology based DNA shuffling. ViroByte strategy is based on BioByte protocol which have been modified in order to enable for randomized fragment assembly.


ViroBytes Assembly Scheme

Retrieved from "http://2010.igem.org/Team:Heidelberg/Notebook/Capsid_Shuffling/ViroBytes"