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Team:Wisconsin-Madison/parts
From 2010.igem.org
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* Gels from cloning and confirmation (.pdf) | * Gels from cloning and confirmation (.pdf) | ||
* All associated testing files and Results (.xls, .pdf, etc.) | * All associated testing files and Results (.xls, .pdf, etc.) | ||
| + | |||
| + | Here is a copy of our submitted standard, BBF-RFC-64: [[Media:BBF_RFC_64.pdf]] | ||
==Enzyme Delivery== | ==Enzyme Delivery== | ||
| Line 48: | Line 50: | ||
|Regulatory - pH inducible promoter | |Regulatory - pH inducible promoter | ||
|<partinfo>BBa_k318513</partinfo> | |<partinfo>BBa_k318513</partinfo> | ||
| - | | | + | |[[media:BBa_K318512_pSB1C3.zip|here]] |
|- | |- | ||
|<partinfo>BBa_k318514</partinfo> | |<partinfo>BBa_k318514</partinfo> | ||
| Line 74: | Line 76: | ||
|Produces Trascription Factor RcsB | |Produces Trascription Factor RcsB | ||
|Inducible - IPTG | |Inducible - IPTG | ||
| - | | | + | |[[media:BBa_K318500_pSB1C3.zip|here]] |
|- | |- | ||
|<partinfo>BBa_k318501</partinfo> | |<partinfo>BBa_k318501</partinfo> | ||
|Produces Trascription Factor RcsA | |Produces Trascription Factor RcsA | ||
|Inducible - IPTG | |Inducible - IPTG | ||
| - | | | + | |[[media:BBa_K318501_pSB1C3.zip|here]] |
|- | |- | ||
|<partinfo>BBa_k318502</partinfo> | |<partinfo>BBa_k318502</partinfo> | ||
|Produces Trascription Factor RcsA & RcsB | |Produces Trascription Factor RcsA & RcsB | ||
|Inducible - IPTG | |Inducible - IPTG | ||
| - | | | + | |[[media:BBa_K318502_pSB1C3.zip|here]] |
|- | |- | ||
|<partinfo>BBa_k318503</partinfo> | |<partinfo>BBa_k318503</partinfo> | ||
|Produces Trascription Factor YgiV - Blocks Biofilm | |Produces Trascription Factor YgiV - Blocks Biofilm | ||
|Inducible - IPTG | |Inducible - IPTG | ||
| - | | | + | |DNA Not Sent |
|- | |- | ||
|<partinfo>BBa_k318504</partinfo> | |<partinfo>BBa_k318504</partinfo> | ||
|Produces RfaI gene - WaaI Ligase | |Produces RfaI gene - WaaI Ligase | ||
|Inducible - IPTG | |Inducible - IPTG | ||
| - | | | + | |DNA Not Sent |
|- | |- | ||
|<partinfo>BBa_k318505</partinfo> | |<partinfo>BBa_k318505</partinfo> | ||
|Produces T.F. YgiV & RfaI gene | |Produces T.F. YgiV & RfaI gene | ||
|Inducible - IPTG | |Inducible - IPTG | ||
| - | | | + | |DNA Not Sent |
|- | |- | ||
|<partinfo>BBa_k318506</partinfo> | |<partinfo>BBa_k318506</partinfo> | ||
|Produces RFP | |Produces RFP | ||
|(+)LuxR, (-)cILVA | |(+)LuxR, (-)cILVA | ||
| - | | | + | |[[media:BBa_K318506_pSB1C3.zip|here]] |
|- | |- | ||
|<partinfo>BBa_k318509</partinfo> | |<partinfo>BBa_k318509</partinfo> | ||
|Produces Repressor cI-LVA (degredation tag) | |Produces Repressor cI-LVA (degredation tag) | ||
|Inducible - IPTG | |Inducible - IPTG | ||
| - | | | + | |DNA Not Sent |
|- | |- | ||
|<partinfo>BBa_k318510</partinfo> | |<partinfo>BBa_k318510</partinfo> | ||
|Produces Repressor cI (no degredation tag) | |Produces Repressor cI (no degredation tag) | ||
|Inducible - IPTG | |Inducible - IPTG | ||
| - | | | + | |DNA Not Sent |
|- | |- | ||
|<partinfo>BBa_k318511</partinfo> | |<partinfo>BBa_k318511</partinfo> | ||
|Produces Activator LuxR | |Produces Activator LuxR | ||
|Anhydrous Tetrycycline | |Anhydrous Tetrycycline | ||
| - | | | + | |[[media:BBa_K318511_pSB1C3.zip|here]] |
|- | |- | ||
|<partinfo>BBa_k318513</partinfo> | |<partinfo>BBa_k318513</partinfo> | ||
|Produces RFP | |Produces RFP | ||
|pH | |pH | ||
| - | | | + | |[[media:BBa_K318513_pSB1C3.zip|here]] |
|- | |- | ||
|<partinfo>BBa_k318515</partinfo> | |<partinfo>BBa_k318515</partinfo> | ||
| Line 146: | Line 148: | ||
|'''Expression Type''' | |'''Expression Type''' | ||
|- | |- | ||
| - | | | + | |Not Cloned |
|Produces Trascription Factors RcsA & RcsB, and B-Gal | |Produces Trascription Factors RcsA & RcsB, and B-Gal | ||
|Constitutive | |Constitutive | ||
|- | |- | ||
| - | | | + | |Not Cloned |
|A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA) | |A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA) | ||
|pH 2 | |pH 2 | ||
|- | |- | ||
|<partinfo>BBa_k318507</partinfo> | |<partinfo>BBa_k318507</partinfo> | ||
| - | |A) Activates Lysis once in the small intestine | + | |A) Activates Lysis once in the small intestine [[media:BBa_K318507_pSB1C3.zip|here]] |
|(+)-LuxR, (-)-cILVA | |(+)-LuxR, (-)-cILVA | ||
|- | |- | ||
| Line 183: | Line 185: | ||
|Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) | |Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) | ||
|[[Media:BBa_K318030_pSB1C3.zip]] | |[[Media:BBa_K318030_pSB1C3.zip]] | ||
| + | |- | ||
| + | |<partinfo>BBa_K318521</partinfo> | ||
| + | |Lock Lysis Cassette - for use with iDIET | ||
| + | |Requires successive pH then bile sensing by the organism to initiate cellular lysis. | ||
| + | |Design phase only | ||
|} | |} | ||
<br> | <br> | ||
Latest revision as of 03:16, 28 October 2010
New Standards
Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:
- Genbank including features and plasmid (.gbk)
- Fwd and Reverse sequencing files (.ab1)
- Gels from cloning and confirmation (.pdf)
- All associated testing files and Results (.xls, .pdf, etc.)
Here is a copy of our submitted standard, BBF-RFC-64: Media:BBF_RFC_64.pdf
Enzyme Delivery
New DNA
| Part Number | About | Testing in... | Zip File |
| <partinfo>BBa_k318512</partinfo> | Regulatory - pH inducible promoter | <partinfo>BBa_k318513</partinfo> | here |
| <partinfo>BBa_k318514</partinfo> | Gene - RamA protein can bind to cholic acid (bile salts) | <partinfo>BBa_k318515</partinfo> | Media:Zip_file_ramA.zip |
| <partinfo>BBa_k318516</partinfo> | Regulatory - RamA/Bile-salt conjugate inducible promoter | <partinfo>BBa_k318517</partinfo> | Media:Zip_file_acrRA.zip |
Testing Constructs
| Part Number | Function | Expression Type | Zip File |
| <partinfo>BBa_k318500</partinfo> | Produces Trascription Factor RcsB | Inducible - IPTG | here |
| <partinfo>BBa_k318501</partinfo> | Produces Trascription Factor RcsA | Inducible - IPTG | here |
| <partinfo>BBa_k318502</partinfo> | Produces Trascription Factor RcsA & RcsB | Inducible - IPTG | here |
| <partinfo>BBa_k318503</partinfo> | Produces Trascription Factor YgiV - Blocks Biofilm | Inducible - IPTG | DNA Not Sent |
| <partinfo>BBa_k318504</partinfo> | Produces RfaI gene - WaaI Ligase | Inducible - IPTG | DNA Not Sent |
| <partinfo>BBa_k318505</partinfo> | Produces T.F. YgiV & RfaI gene | Inducible - IPTG | DNA Not Sent |
| <partinfo>BBa_k318506</partinfo> | Produces RFP | (+)LuxR, (-)cILVA | here |
| <partinfo>BBa_k318509</partinfo> | Produces Repressor cI-LVA (degredation tag) | Inducible - IPTG | DNA Not Sent |
| <partinfo>BBa_k318510</partinfo> | Produces Repressor cI (no degredation tag) | Inducible - IPTG | DNA Not Sent |
| <partinfo>BBa_k318511</partinfo> | Produces Activator LuxR | Anhydrous Tetrycycline | here |
| <partinfo>BBa_k318513</partinfo> | Produces RFP | pH | here |
| <partinfo>BBa_k318515</partinfo> | Produces RFP | RamA/Bile-Salt Complex | Media:Zip_file_acrRA.zip |
| <partinfo>BBa_k318517</partinfo> | Produces RamA gene | Bile Salt | Media:Zip_file_ramA.zip |
Final Constructs
| Part Number | Function | Expression Type |
| Not Cloned | Produces Trascription Factors RcsA & RcsB, and B-Gal | Constitutive |
| Not Cloned | A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA) | pH 2 |
| <partinfo>BBa_k318507</partinfo> | A) Activates Lysis once in the small intestine here | (+)-LuxR, (-)-cILVA |
| <partinfo>BBa_k318519</partinfo> | B) Activates lysis once in the small intestine | Bile Salts |
Encryption
| Part Number | Description | Notes | Zip File |
| <partinfo>BBa_K318000</partinfo> | Key Cassette - produces Cre and Hin recombinases | Arabinose induces Cre expression, IPTG induces Hin expression | Media:BBa_K318000_pSB1C3.zip |
| <partinfo>BBa_K318030</partinfo> | Lock Cassette - prevents transcription unless "unlocked" | Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) | Media:BBa_K318030_pSB1C3.zip |
| <partinfo>BBa_K318521</partinfo> | Lock Lysis Cassette - for use with iDIET | Requires successive pH then bile sensing by the organism to initiate cellular lysis. | Design phase only |
Other Parts
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318010 BBa_K318010]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318011 BBa_K318011]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318012 BBa_K318012]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318013 BBa_K318013]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318014 BBa_K318014]
