Team:NYMU-Taipei/Applications

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=Your promoter and Your protein=
=Your promoter and Your protein=
[[Image:NYMU main circuit.png‎|thumb|500px|right|Project overview by figure.]]
[[Image:NYMU main circuit.png‎|thumb|500px|right|Project overview by figure.]]
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In the figure of our project overview, you can see '''your promoter''' and '''your protein''' there. Because of that the main purpose of our project is provide a speedy and useful tool for everyone who is doing synthetic biology, we designed a flexible tool which can applicative in different needs. The explanation of your promoter and your protein is as below:
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In the overview figure of our project, you can see '''your promoter''' and '''your protein'''. Because the main purpose of our project is to provide a speedy and useful tool for everyone who is doing synthetic biology research, we designed a flexible system which can be applied for different needs. The explanation of your promoter and your protein is as below:
*Your promoter
*Your promoter
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Here you can change your own promoter which you are interesting. This part can used to measure the quantitative and locations of mRNA induced by different types or different strength promoter. With this novel method, we can faster and more understanding the relationship between promoters and mRNA transcription.
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Here you can change "your promoter" with your own promoter which you are interested in. This part can be used to measure the quantity and locations of mRNA induced by different types or different strength promoters. With this novel method, we can get faster and more understanding the relationship between promoters and mRNA transcription.
*Your protein
*Your protein
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Here you can also change your own protein and fusion with our specific peptide adaptor. Then you can use the novel speedy protein reporter in our SpeedyBac to know the localization of different proteins and the influence of translation by different situations.
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Here you can also change to the use of your own protein and by fusion with our specific peptide adaptor. Then you can use the novel speedy protein reporter in our SpeedyBac system to know the localization of different proteins and the influence of translation under different situations.
=Applications=
=Applications=
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Our project can achieve several benefits and have several applications as below:
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Our project has several benefits and have several applications as follows:
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*Our SpeedyBac can be used to measure the gene expression in single cell, it help us to avoid the influence from population.
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*Our SpeedyBac can be used to measure the gene expression in single cells. It helps us to avoid some potential pitfalls resulted in bulk assays (Muzzey and van Oudenaarden, 2009).
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*Users can change their own promoters and proteins easily and measure them faster than traditional methods.
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*Users can exchange our promoter and protein for their own easily and measure them much faster than traditional methods.
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*With our Speedy switch, we can stop the gene expression at transcription, so we can study the roles of mRNA in gene expression more easily.
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*With our Speedy switch, we can halt the gene expression at transcription level, so we can study the roles of mRNA in gene expression more easily.
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*Compared to the conventional methods for measuring gene expression, our SpeedyBac provide a faster and more flexible way in studying the gene expression and interactions between different biological parts in ''vivo''.
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=Reference=
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Muzzey, D., and van Oudenaarden, A. (2009). Quantitative time-lapse fluorescence microscopy in single cells. Annu Rev Cell Dev Biol 25, 301-327.
{{:Team:NYMU-Taipei/Footer}}
{{:Team:NYMU-Taipei/Footer}}

Latest revision as of 02:35, 28 October 2010

Your promoter and Your protein

Project overview by figure.

In the overview figure of our project, you can see your promoter and your protein. Because the main purpose of our project is to provide a speedy and useful tool for everyone who is doing synthetic biology research, we designed a flexible system which can be applied for different needs. The explanation of your promoter and your protein is as below:

  • Your promoter

Here you can change "your promoter" with your own promoter which you are interested in. This part can be used to measure the quantity and locations of mRNA induced by different types or different strength promoters. With this novel method, we can get faster and more understanding the relationship between promoters and mRNA transcription.

  • Your protein

Here you can also change to the use of your own protein and by fusion with our specific peptide adaptor. Then you can use the novel speedy protein reporter in our SpeedyBac system to know the localization of different proteins and the influence of translation under different situations.

Applications

Our project has several benefits and have several applications as follows:

  • Our SpeedyBac can be used to measure the gene expression in single cells. It helps us to avoid some potential pitfalls resulted in bulk assays (Muzzey and van Oudenaarden, 2009).
  • Users can exchange our promoter and protein for their own easily and measure them much faster than traditional methods.
  • With our Speedy switch, we can halt the gene expression at transcription level, so we can study the roles of mRNA in gene expression more easily.
  • Compared to the conventional methods for measuring gene expression, our SpeedyBac provide a faster and more flexible way in studying the gene expression and interactions between different biological parts in vivo.

Reference

Muzzey, D., and van Oudenaarden, A. (2009). Quantitative time-lapse fluorescence microscopy in single cells. Annu Rev Cell Dev Biol 25, 301-327.

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