Team:Panama/21 October 2010
From 2010.igem.org
(Difference between revisions)
(→October 21) |
(→October 21) |
||
(10 intermediate revisions not shown) | |||
Line 6: | Line 6: | ||
0.8% agarose, 1x TBE, 1.5 ul EtBr | 0.8% agarose, 1x TBE, 1.5 ul EtBr | ||
- | [[Image: | + | [[Image:Cuadro21octubre1.JPG|550px|thumb|left|alt text]] |
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | [[Image:UntitledGel21Octubre3_2.JPG|200px|thumb|left|alt text]] | ||
Line 26: | Line 37: | ||
1) PCR sample 6 | 1) PCR sample 6 | ||
+ | |||
[[Image:UntitledPCR_sample6_Oct21.JPG|200px|thumb|left|alt text]] | [[Image:UntitledPCR_sample6_Oct21.JPG|200px|thumb|left|alt text]] | ||
Line 35: | Line 47: | ||
- | [[Image: | + | |
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | '''Results''' | ||
+ | [[Image:Cuadro21octubre3.JPG|300px|thumb|left|alt text]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | [[Image:UntitledOct21.JPG|200px|thumb|left|alt text]] | ||
+ | |||
+ | |||
+ | We used to digest with Pstl the colony #3 obtained from quick screening on 20 October, as the band profiles obtained from our gene seemed possessed Rh1AB. | ||
+ | |||
+ | |||
+ | |||
Latest revision as of 02:20, 28 October 2010
October 21
Electrophoresis: Miniprep Samples 0.8% agarose, 1x TBE, 1.5 ul EtBr
Sample 6 is a possible plasmid with insert
1) PCR sample 6
Results
We used to digest with Pstl the colony #3 obtained from quick screening on 20 October, as the band profiles obtained from our gene seemed possessed Rh1AB.
2. Digest with EcoR1
Digestion of Sample 6 with EcoR1 Take 1.21 ul for 100 ng
|
|
|
|
|
|