Team:DTU-Denmark/Parts

From 2010.igem.org

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<h1>BioBricks submitted</h1>
 
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<h3><b>Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR</b></h3>
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<b>BioBricks submitted</b><br><br>
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<p align="justify">The table shows all of our submitted BioBricks and further down we have a more detailed description of the BioBricks that we have characterized</p>
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<div align="center"><groupparts>iGEM010 DTU-Denmark</groupparts></div>
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<b>Characterized BioBricks</b><br><br>
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<h4><b>Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR</b></h4>
<ul><li>BBa_K374008</li></ul>
<ul><li>BBa_K374008</li></ul>
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<p align="justify">This part has been characterized, for more information look <a href="https://2010.igem.org/Team:DTU-Denmark/Repressor_Section#Characterization">here </a> or in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374008" target="_blank"> BBa_K374008 </a></p>
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<p align="justify">For more information look <a href="https://2010.igem.org/Team:DTU-Denmark/Repressor_Section#Characterization">here </a> or in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374008" target="_blank"> BBa_K374008 </a></p>
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<h3><b>Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR</b></h3>
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<h4><b>Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR</b></h4>
<ul><li>BBa_K374009</li></ul>
<ul><li>BBa_K374009</li></ul>
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<p align="justify">This part has been characterized, for more information look <a href="https://2010.igem.org/Team:DTU-Denmark/Repressor_Section#Characterization">here </a> or in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374009" target="_blank"> BBa_K374009 </a></p>
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<p align="justify">For more information look <a href="https://2010.igem.org/Team:DTU-Denmark/Repressor_Section#Characterization">here </a> or in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374009" target="_blank"> BBa_K374009 </a></p>
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<h3><b>Antirepressor AntO from Gifsy1 phage</b></h3>
 
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<ul><li>BBa_K374010</li></ul>
 
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<p align="justify"> AntO inactivates the repressor from Gifsy1 phage, resulting in transcription from the pR<sup>Gifsy1</sup> promoter. AntO works by direct non-covalent binding to gogR and prevents its DNA binding activity.</p>
 
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<p align="justify"><i>This regulatory biobrick can be used in a biological circuit. It can de-repress the effect of the repressor from<a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374008" target="_blank">  BBa_K374008 </a> allowing expression from the pR<sup>Gifsy1</sup> promoter.</i></p>
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<h4><b>N anti-terminator gene from Lambda phage</b></h4>
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<p align="justify">For more information look in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374010" target="_blank"> BBa_K374010 </a></p>
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<h3><b>NutRsite - N anti-terminator utilization site from Lamdba phage</b></h3>
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<ul><li>BBa_K374005</li></ul>
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<p align="justify">In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element. This part contains the lambda nutR site which together with the N-protein will suppress termination downstream regardless of what terminator is used.</p>
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<p align="justify"><i>This regulatory biobrick can be used in a biological circuit. It can supress any termination, if placed upstream of the terminator and applied together with<a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374006" target="_blank">  BBa_K374006 </a> </i></p>
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<p align="justify">For more information look in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374005" target="_blank"> BBa_K374005 </a></p>
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<h3><b>N anti-terminator protein from Lambda phage</b></h3>
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<ul><li>BBa_K374006</li></ul>
<ul><li>BBa_K374006</li></ul>
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<p align="justify"><i>This regulatory biobrick can be used in a biological circuit. Together with<a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374005" target="_blank">  BBa_K374005 </a>, they will promote anti-termination. </i></p>
<p align="justify"><i>This regulatory biobrick can be used in a biological circuit. Together with<a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374005" target="_blank">  BBa_K374005 </a>, they will promote anti-termination. </i></p>
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<p align="justify">For more information look in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374006" target="_blank"> BBa_K374006 </a></p>
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<p align="justify">For more information look <a href="https://2010.igem.org/Team:DTU-Denmark/Repressor_Section#Characterization">here</a> or in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374006" target="_blank"> BBa_K374006 </a></p>
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<h3><b>NutRsite followed by BioBrick terminator BBa_B0015</b></h3>
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<ul><li>BBa_K374007</li></ul>
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<p align="justify">This is a composite part, consisting of the Lambda nutR site part<a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374005" target="_blank"> BBa_K374005 </a>with the BioBrick terminator <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015" target="_blank"> BBa_B0015 </a>BBa_B0015 downstream of it</p>
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<p align="justify">For more information look in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374007" target="_blank"> BBa_K374007 </a></p>
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<h3><b>N anti-terminator with natural RBS</b></h3>
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<ul><li>BBa_K374011</li></ul>
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<p align="justify">This part contains the lambda N-gene which (also submitted as BioBrick <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374006" target="_blank"> BBa_K374006</a>) with its natural RBS.</p>
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<p align="justify">For more information look in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374011" target="_blank"> BBa_K374011 </a></p>
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<h3><b>Fluorescence Activated Cell Sorting (FACS) optimized GFP</b></h3>
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<ul><li>BBa_K374012</li></ul>
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<p align="justify">FACS optimized mutant of the Green Flourescent Protein. The protein contains the following three mutations: S65A, V68L and S72A. Excitation maximum at 488 nm.</p>
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<p align="justify">For more information see <a href=" http://ip.com/patent/US5804387 " target="_blank"> US Patent 5,804,387 </a> or parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374012" target="_blank"> BBa_K374012 </a>  </p>
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<h3><b>Lambda N-gene followed by BioBrick RFP BBa_E1010</b></h3>
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<ul><li>BBa_K374013</li></ul>
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<p align="justify">This part is a composite part which contains the lambda N-gene (<a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374006" target="_blank"> BBa_K374006 </a>) with its natural RBS followed by a BioBrick RBS <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034" target="_blank"> BBa_B0034 </a> and RFP <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010" target="_blank"> BBa_E1010 </a>.</p>
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<p align="justify">For more information look in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374013" target="_blank"> BBa_K374013 </a></p>
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=== Parts ===
 
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
 
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<groupparts>iGEM010 DTU-Denmark</groupparts>
 

Latest revision as of 02:14, 28 October 2010

Welcome to the DTU iGEM wiki!

Home The Team The Project Parts submitted Results Notebook Blog


BioBricks submitted

The table shows all of our submitted BioBricks and further down we have a more detailed description of the BioBricks that we have characterized
<groupparts>iGEM010 DTU-Denmark</groupparts>


Characterized BioBricks

Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR

  • BBa_K374008

This part contains the promoters: pRGifsy1 and pRMGifsy1. Downstream of the pRMGifsy1 promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRMGifsy1 and a repressor of the pRGifsy1.

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GogR repressor. BBa_K374008 can be combined with BBa_K374010 hence allowing expression of your inserted BioBrick.

For more information look here or in parts-registry, BBa_K374008

Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR

  • BBa_K374009

This part contains the promoters: pRGifsy2 and pRMGifsy2. Downstream of the pRMGifsy2 promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRMGifsy2 and a repressor of the pRGifsy2.

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick.

For more information look here or in parts-registry, BBa_K374009

N anti-terminator gene from Lambda phage

  • BBa_K374006

In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element. This part contains the lambda N gene which will suppress transcription termination downstream of the nutR site.

This regulatory biobrick can be used in a biological circuit. Together with BBa_K374005 , they will promote anti-termination.

For more information look here or in parts-registry, BBa_K374006

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