Team:DTU-Denmark/Parts

From 2010.igem.org

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<h1>BioBricks submitted</h1>
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<p align="justify">BBa_K374008</p>
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<font size="3"><b>Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR</b></font><br>
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<p align="justify"><i>This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GogR repressor. BBa_K374008 can be combined with BBa_K3740010 hence allowing expression of your inserted BioBrick.</i></p>
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<b>BioBricks submitted</b><br><br>
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<p align="justify">The table shows all of our submitted BioBricks and further down we have a more detailed description of the BioBricks that we have characterized</p>
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<div align="center"><groupparts>iGEM010 DTU-Denmark</groupparts></div>
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<b>Characterized BioBricks</b><br><br>
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<h4><b>Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR</b></h4>
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<ul><li>BBa_K374008</li></ul>
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<p align="justify"> This part contains the promoters: pR<sup>Gifsy1</sup> and pRM<sup>Gifsy1</sup>. Downstream of the pRM<sup>Gifsy1</sup> promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM<sup>Gifsy1</sup> and a repressor of the pR<sup>Gifsy1</sup>.</p>
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<p align="justify"><i>This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GogR repressor. BBa_K374008 can be combined with <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374010" target="_blank"> BBa_K374010 </a>hence allowing expression of your inserted BioBrick.</i></p>
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<p align="justify">Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p>
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<p align="justify">For more information look <a href="https://2010.igem.org/Team:DTU-Denmark/Repressor_Section#Characterization">here </a> or in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374008" target="_blank"> BBa_K374008 </a></p>
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Reference
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<p align="justify">BBa_K374009
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<p align="justify">Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR</p>
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<h4><b>Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR</b></h4>
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<p align="justify">Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p>
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<ul><li>BBa_K374009</li></ul>
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<p align="justify">This part contains the promoters: pR<sup>Gifsy2</sup> and pRM<sup>Gifsy2</sup>. Downstream of the pRM<sup>Gifsy2</sup> promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM<sup>Gifsy2</sup> and a repressor of the pR<sup>Gifsy2</sup>.</p>
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<p align="justify"><i>This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick.</i></p>
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<p align="justify">For more information look <a href="https://2010.igem.org/Team:DTU-Denmark/Repressor_Section#Characterization">here </a> or in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374009" target="_blank"> BBa_K374009 </a></p>
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<h4><b>N anti-terminator gene from Lambda phage</b></h4>
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<ul><li>BBa_K374006</li></ul>
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<p align="justify">In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element. This part contains the lambda N gene which will suppress transcription termination downstream of  
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the nutR site.</p>
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<p align="justify"><i>This regulatory biobrick can be used in a biological circuit. Together with<a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374005" target="_blank">  BBa_K374005 </a>, they will promote anti-termination. </i></p>
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<p align="justify">For more information look <a href="https://2010.igem.org/Team:DTU-Denmark/Repressor_Section#Characterization">here</a> or in parts-registry, <a href=" http://partsregistry.org/wiki/index.php?title=Part:BBa_K374006" target="_blank"> BBa_K374006 </a></p>
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=== Parts ===
 
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
 
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<groupparts>iGEM010 DTU-Denmark</groupparts>
 

Latest revision as of 02:14, 28 October 2010

Welcome to the DTU iGEM wiki!

Home The Team The Project Parts submitted Results Notebook Blog


BioBricks submitted

The table shows all of our submitted BioBricks and further down we have a more detailed description of the BioBricks that we have characterized
<groupparts>iGEM010 DTU-Denmark</groupparts>


Characterized BioBricks

Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR

  • BBa_K374008

This part contains the promoters: pRGifsy1 and pRMGifsy1. Downstream of the pRMGifsy1 promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRMGifsy1 and a repressor of the pRGifsy1.

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GogR repressor. BBa_K374008 can be combined with BBa_K374010 hence allowing expression of your inserted BioBrick.

For more information look here or in parts-registry, BBa_K374008

Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR

  • BBa_K374009

This part contains the promoters: pRGifsy2 and pRMGifsy2. Downstream of the pRMGifsy2 promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRMGifsy2 and a repressor of the pRGifsy2.

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick.

For more information look here or in parts-registry, BBa_K374009

N anti-terminator gene from Lambda phage

  • BBa_K374006

In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element. This part contains the lambda N gene which will suppress transcription termination downstream of the nutR site.

This regulatory biobrick can be used in a biological circuit. Together with BBa_K374005 , they will promote anti-termination.

For more information look here or in parts-registry, BBa_K374006

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