Team:DTU-Denmark

From 2010.igem.org

(Difference between revisions)
 
(36 intermediate revisions not shown)
Line 1: Line 1:
-
[[image:wiki banner_967px.png]]
 
-
 
<html>
<html>
 +
<img src="https://static.igem.org/mediawiki/2010/6/6b/Wiki_banner_967px.png"  align="center"> </img>
<head>
<head>
<title>Welcome to the DTU iGEM wiki!</title>
<title>Welcome to the DTU iGEM wiki!</title>
Line 42: Line 41:
.links tr {
.links tr {
-
  background-color:#660066;
+
  background-color:#990000;
  height:30px;
  height:30px;
}
}
Line 66: Line 65:
.links a:hover {
.links a:hover {
-
background-color: #660066;
+
background-color: #999999;
}
}
Line 125: Line 124:
  background-color: #DDDDDD;
  background-color: #DDDDDD;
}
}
-
 
+
caption {font-size:85%}
</style>
</style>
<! -- END of style sheet -->
<! -- END of style sheet -->
Line 140: Line 139:
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Project" >The Project</a> </font></td>
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Project" >The Project</a> </font></td>
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Parts" >Parts submitted</a> </font></td>
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Parts" >Parts submitted</a> </font></td>
-
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Modelling">Modelling</a></font> </td>
+
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Results">Results</a></font> </td>
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Notebook" title="Day to day lab activity">Notebook</a>  
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Notebook" title="Day to day lab activity">Notebook</a>  
   <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Blog">Blog</a></font> </td>
   <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Blog">Blog</a></font> </td>
Line 160: Line 159:
     Sponsors and acknowledgements<br><br>
     Sponsors and acknowledgements<br><br>
   <font color="DDDDDD" face="arial" size="2">
   <font color="DDDDDD" face="arial" size="2">
-
     <a href="https://2009.igem.org/Team:DTU_Denmark/sponsors" CLASS=leftbar>- List of sponsors</a><br>
+
<ul>
-
     <a href="https://2010.igem.org/Team:DTU-Denmark/Acknowledgement" CLASS=leftbar>- Acknowledgements</a><br>
+
     <li><a href="https://2010.igem.org/Team:DTU-Denmark/sponsors" CLASS=leftbar>List of sponsors</a></li>
 +
     <li><a href="https://2010.igem.org/Team:DTU-Denmark/Acknowledgement" CLASS=leftbar>Acknowledgements and Contributions</a></li>
 +
    <li><a href="https://2010.igem.org/Team:DTU-Denmark/Collaborations" CLASS=leftbar>Collaborations</a></li>
 +
</ul>
   </font>
   </font>
<br>
<br>
Line 169: Line 171:
   </font>
   </font>
   <font color="DDDDDD" face="arial" size="2">
   <font color="DDDDDD" face="arial" size="2">
-
     <a href="http://www.bio.dtu.dk/igem.aspx" target="_blank" CLASS=leftbar>- DTU iGEM 2010</a><br>
+
<ul>
 +
     <li><a href="http://www.bio.dtu.dk/igem.aspx" target="_blank" CLASS=leftbar>DTU iGEM 2010</a></li>
 +
</ul>
     <br>
     <br>
   </font>
   </font>
Line 177: Line 181:
   </font>
   </font>
   <font color="DDDDDD" face="arial" size="2">
   <font color="DDDDDD" face="arial" size="2">
-
     <a href="http://www.facebook.com/pages/iGEM-2010-DTU/134540923224394?ref=ts" target="_blank" CLASS=leftbar>- Facebook</a><br>
+
<ul>
 +
     <li><a href="http://www.facebook.com/pages/iGEM-2010-DTU/134540923224394?ref=ts" target="_blank" CLASS=leftbar>Facebook</a></li>
 +
    <li><a href="http://twitter.com/igemdtu2010" target="_blank" CLASS=leftbar>Twitter</a></li>
 +
</ul>
 +
    <br>
 +
  </font>
 +
<font color="#990000" face="arial" size="3">
 +
<br>
 +
Our Galllery:<br><br>
   </font>
   </font>
   <font color="DDDDDD" face="arial" size="2">
   <font color="DDDDDD" face="arial" size="2">
-
     <a href="http://twitter.com/igemdtu2010" target="_blank" CLASS=leftbar>- Twitter</a><br>
+
<ul>
-
    <br>
+
     <li><a href="https://2010.igem.org/Team:DTU-Denmark/Gallery">Photo Gallery</a></li>
 +
</ul>
   </font>
   </font>
   </td>
   </td>
Line 189: Line 202:
<b>DTU DENMARK</b><br><br>
<b>DTU DENMARK</b><br><br>
   </font>
   </font>
-
  <font color="#333333" face="arial" size="2.5">
+
<p align="justify">The aim of this project is to engineer a genetic bistable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the Salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.</p>
-
 
+
<p align="justify">We present the framework for this development and we characterize the regulatory mechanisms by using fluorescent proteins as outputs. The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the Salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.</p>
 +
<p align="justify">There are multiple potential applications for a biological "switch" like ours in the world of biotechnology i.e. within the medical and environmental field.</p>
-
<!-- INSERT MAIN TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
+
<table class="https://static.igem.org/mediawiki/2010/e/ec/DTU_2biostable.png" align="center">
-
 
+
<caption align="bottom"><p align="justify"><b>Figure 1</b> Fun in the lab!!</p></caption>
-
<p align="justify">The aim of this project is to engineer a genetic bi-stable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.</p>
+
<tr><td><img src="https://static.igem.org/mediawiki/2010/e/ec/DTU_2biostable.png" width="300px"></td></tr>
-
<p align="justify">We present the framework for this development and characterize the regulatory mechanisms by using fluorescent proteins as the reporter (outputs). The dynamics of the system have been modeled and we have also attempted to characterize and submit the promoters, repressors and anti-repressors from the salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.</p>
+
</table><br>
-
<h3>Basic Concept</h3>
+
<table align="center">
-
<img src="https://static.igem.org/mediawiki/2010/f/ff/DTU_Project_illustration_1.png" width="570px" align="center"> </img>  
+
<tr>
-
<p align="left"><font size="1">Figure 1</font></p><br>
+
<td align="center">
-
<p align="justify">Figure 1 shows the basic concept of our project:<br><i>Inducer 1</i> will activate <i>Promoter 1</i> thereby start up the production of <i>Repressor 1</i>, switching off the other part of our switch by repressing <i>Promoter 2</i>. This will essentially make the cells change from <font color="#990000">red</font color> to <font color="#33FF00">green</font color>!</p>
+
<table class="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif" align="center">
-
<p align="justify">This means that when the cells are exposed to <i>Inducer 2</i>, the cell culture will turn from <font color="#33FF00">green</font color> to <font color="#990000">red</font color>.</p>
+
<tr><td><img src="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif"  width="150px"></td></tr>
-
  </font>
+
</table><br>
 +
</td>
 +
<td align="center">
 +
<table class="https://static.igem.org/mediawiki/2010/9/93/DTU_Corrit_fonden.png" align="center">
 +
<tr><td><img src="https://static.igem.org/mediawiki/2010/9/93/DTU_Corrit_fonden.png"  width="150px"></td></tr>
 +
</table><br>
 +
</td>
 +
<td align="center">
 +
<table class="https://static.igem.org/mediawiki/2010/8/80/DTUlogo.png" align="center">
 +
<tr><td><img src="https://static.igem.org/mediawiki/2010/8/80/DTUlogo.png" ></td></tr>
 +
</table><br>
 +
</tr>
 +
</table>
  </td>
  </td>
  <td width="182px" height="100%" valign="top" class="greybox">
  <td width="182px" height="100%" valign="top" class="greybox">
Line 212: Line 238:
<font size="3"><strong>Quick Update</strong></font><br>
<font size="3"><strong>Quick Update</strong></font><br>
<br>
<br>
 +
 +
<strong>October 24th, 2010</strong><br>
 +
<p>The structure of our wiki has been changed slightly... Our <b><a href="https://2010.igem.org/Team:DTU-Denmark/Modelling"  target="_blank">Modeling</a></b> section can now be found under <b><a href="https://2010.igem.org/Team:DTU-Denmark/Project"  target="_blank">The Project</a></b>.<br>
 +
<br>
 +
</p>
 +
 +
<strong>October 15th, 2010</strong><br>
 +
<p>Maya, Thomas and Juliet took the BioLector for a ride! And it was cool way to spend our Friday evening ;D<br>
 +
<br>
 +
</p>
 +
 +
<strong>October 7th, 2010</strong><br>
 +
<p>The DTU-iGEM team will be meeting up with the SDU-iGEM team this Saturday (9th Oct) give each other feedback on our projects and to have a social event!<br>
 +
<br>
 +
</p>
 +
 +
<strong>October 1st, 2010</strong><br>
 +
<p>Check out our active <a href="https://2010.igem.org/Team:DTU-Denmark/Blog">blog</a>: now with picture of the day!<br>
 +
<br>
 +
</p>
<strong>August 14th, 2010</strong><br>
<strong>August 14th, 2010</strong><br>
-
Pictures have been posted so check out our <a href="https://2010.igem.org/Team:DTU-Denmark/Gallery">picture gallery</a>!<br>
+
<p>Pictures have been posted so check out our <a href="https://2010.igem.org/Team:DTU-Denmark/Gallery">picture gallery</a>!<br>
<br>
<br>
</p>
</p>

Latest revision as of 01:52, 28 October 2010

Welcome to the DTU iGEM wiki!

Home The Team The Project Parts submitted Results Notebook Blog

Sponsors and acknowledgements



Also check out our Danish website

Also check us out on:

Our Galllery:
DTU DENMARK

The aim of this project is to engineer a genetic bistable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the Salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.

We present the framework for this development and we characterize the regulatory mechanisms by using fluorescent proteins as outputs. The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the Salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.

There are multiple potential applications for a biological "switch" like ours in the world of biotechnology i.e. within the medical and environmental field.

Figure 1 Fun in the lab!!





Quick Update

October 24th, 2010

The structure of our wiki has been changed slightly... Our Modeling section can now be found under The Project.

October 15th, 2010

Maya, Thomas and Juliet took the BioLector for a ride! And it was cool way to spend our Friday evening ;D

October 7th, 2010

The DTU-iGEM team will be meeting up with the SDU-iGEM team this Saturday (9th Oct) give each other feedback on our projects and to have a social event!

October 1st, 2010

Check out our active blog: now with picture of the day!

August 14th, 2010

Pictures have been posted so check out our picture gallery!
Comments or questions to the team? Please

Retrieved from "http://2010.igem.org/Team:DTU-Denmark"