Team:DTU-Denmark

The aim of this project is to engineer a genetic bistable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the Salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.

We present the framework for this development and we characterize the regulatory mechanisms by using fluorescent proteins as outputs. The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the Salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.

There are multiple potential applications for a biological "switch" like ours in the world of biotechnology i.e. within the medical and environmental field.

**Figure 1** Fun in the lab!!

Quick Update

October 24th, 2010

The structure of our wiki has been changed slightly... Our Modeling section can now be found under The Project.

October 15th, 2010

Maya, Thomas and Juliet took the BioLector for a ride! And it was cool way to spend our Friday evening ;D

October 7th, 2010

The DTU-iGEM team will be meeting up with the SDU-iGEM team this Saturday (9th Oct) give each other feedback on our projects and to have a social event!

October 1st, 2010

Check out our active blog: now with picture of the day!

August 14th, 2010

Pictures have been posted so check out our picture gallery!

Comments or questions to the team? Please Email us

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+<img src="https://static.igem.org/mediawiki/2010/6/6b/Wiki_banner_967px.png"  align="center"> </img>
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 <title>Welcome to the DTU iGEM wiki!</title>
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      <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Project" >The Project</a> </font></td>
      <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Parts" >Parts submitted</a> </font></td>
-     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Modelling">Modelling</a></font> </td>
+     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Results">Results</a></font> </td>
      <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Notebook" title="Day to day lab activity">Notebook</a>
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Blog">Blog</a></font> </td>
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      Sponsors and acknowledgements<br><br>
     <font color="DDDDDD" face="arial" size="2">
-     <a href="https://2009.igem.org/Team:DTU_Denmark/sponsors" CLASS=leftbar>- List of sponsors</a><br>
+<ul>
-     <a href="https://2009.igem.org/Team:DTU_Denmark/acknowledgement" CLASS=leftbar>- Acknowledgement</a><br>
+     <li><a href="https://2010.igem.org/Team:DTU-Denmark/sponsors" CLASS=leftbar>List of sponsors</a></li>
+     <li><a href="https://2010.igem.org/Team:DTU-Denmark/Acknowledgement" CLASS=leftbar>Acknowledgements and Contributions</a></li>
+    <li><a href="https://2010.igem.org/Team:DTU-Denmark/Collaborations" CLASS=leftbar>Collaborations</a></li>
+</ul>
     </font>
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     </font>
     <font color="DDDDDD" face="arial" size="2">
-     <a href="http://www.bio.dtu.dk/igem.aspx" target="_blank" CLASS=leftbar>- DTU iGEM 2010</a><br>
+<ul>
+     <li><a href="http://www.bio.dtu.dk/igem.aspx" target="_blank" CLASS=leftbar>DTU iGEM 2010</a></li>
+</ul>
      <br>
+   </font>
+<font color="#990000" face="arial" size="3">
+<br>
+ Also check us out on:<br><br>
+   </font>
+   <font color="DDDDDD" face="arial" size="2">
+<ul>
+    <li><a href="http://www.facebook.com/pages/iGEM-2010-DTU/134540923224394?ref=ts" target="_blank" CLASS=leftbar>Facebook</a></li>
+    <li><a href="http://twitter.com/igemdtu2010" target="_blank" CLASS=leftbar>Twitter</a></li>
+</ul>
+    <br>
+   </font>
+<font color="#990000" face="arial" size="3">
+<br>
+ Our Galllery:<br><br>
+   </font>
+   <font color="DDDDDD" face="arial" size="2">
+<ul>
+    <li><a href="https://2010.igem.org/Team:DTU-Denmark/Gallery">Photo Gallery</a></li>
+</ul>
     </font>
    </td>
@@ Line 178: / Line 202: @@
 <b>DTU DENMARK</b><br><br>
    </font>
-  <font color="#333333" face="arial" size="2.5">
+<p align="justify">The aim of this project is to engineer a genetic bistable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the Salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.</p>
+<p align="justify">We present the framework for this development and we characterize the regulatory mechanisms by using fluorescent proteins as outputs. The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the Salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.</p>
+<p align="justify">There are multiple potential applications for a biological "switch" like ours in the world of biotechnology i.e. within the medical and environmental field.</p>
-<!-- INSERT MAIN TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
+<table class="https://static.igem.org/mediawiki/2010/e/ec/DTU_2biostable.png" align="center">
+ <caption align="bottom"><p align="justify"><b>Figure 1</b> Fun in the lab!!</p></caption>
-<p align="justify">Greetings from Team DTU!<br>
+<tr><td><img src="https://static.igem.org/mediawiki/2010/e/ec/DTU_2biostable.png"  width="300px"></td></tr>
-Our team is made up of 10 students competing in this years iGEM competition. This year's team representing <a href="http://www.dtu.dk/English/About_DTU.aspx">DTU</a> is made up of a diverse group of students, both culturally and scientifically. This means that we get to learn so much from each other, professionally as well as culturally. As we are studying at a technical University, we are given an even greater opportunity to really flex our brain-muscles and be innovative. <i>You can also follow us on <b><a href="http://www.facebook.com/pages/iGEM-2010-DTU/134540923224394?ref=ts">Facebook</a></b> and <b><a href="http://twitter.com/igemdtu2010">Twitter</a></b>!</i>
+</table><br>
+<table align="center">
-<p align="justify">The goal of our project is to enable colonies of E. coli bacteria to transition between production of two different reporter proteins. In our system, switching between states will be induced by exposing the bacteria to light. Each of the states will have a specific frequency associated with it. There are multiple potential applications for biologicals "switches" such as these, this includes the improved control of production of additives in industrial biotechnological processes. For more details, please click <a href="https://2010.igem.org/wiki/Team:DTU-Denmark/Project">here</a>.
+<tr>
+<td align="center">
-<p align="justify"><img src="https://static.igem.org/mediawiki/2010/7/78/DTU_paris.jpg" width="570px"  align="left"> The team in Paris for the iGEM workshop (Malte is missing)</img>
+<table class="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif" align="center">
-  </font>
+<tr><td><img src="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif"  width="150px"></td></tr>
+</table><br>
+</td>
+<td align="center">
+<table class="https://static.igem.org/mediawiki/2010/9/93/DTU_Corrit_fonden.png" align="center">
+<tr><td><img src="https://static.igem.org/mediawiki/2010/9/93/DTU_Corrit_fonden.png"  width="150px"></td></tr>
+</table><br>
+</td>
+<td align="center">
+<table class="https://static.igem.org/mediawiki/2010/8/80/DTUlogo.png" align="center">
+<tr><td><img src="https://static.igem.org/mediawiki/2010/8/80/DTUlogo.png" ></td></tr>
+</table><br>
+</tr>
+</table>
   </td>
   <td width="182px" height="100%" valign="top" class="greybox">
@@ Line 200: / Line 238: @@
 <font size="3"><strong>Quick Update</strong></font><br>
 <br>
+<strong>October 24th, 2010</strong><br>
+<p>The structure of our wiki has been changed slightly... Our <b><a href="https://2010.igem.org/Team:DTU-Denmark/Modelling"  target="_blank">Modeling</a></b> section can now be found under <b><a href="https://2010.igem.org/Team:DTU-Denmark/Project"  target="_blank">The Project</a></b>.<br>
+<br>
+</p>
+<strong>October 15th, 2010</strong><br>
+<p>Maya, Thomas and Juliet took the BioLector for a ride! And it was cool way to spend our Friday evening ;D<br>
+<br>
+</p>
+<strong>October 7th, 2010</strong><br>
+<p>The DTU-iGEM team will be meeting up with the SDU-iGEM team this Saturday (9th Oct) give each other feedback on our projects and to have a social event!<br>
+<br>
+</p>
+<strong>October 1st, 2010</strong><br>
+<p>Check out our active <a href="https://2010.igem.org/Team:DTU-Denmark/Blog">blog</a>: now with picture of the day!<br>
+<br>
+</p>
 <strong>August 14th, 2010</strong><br>
-Pictures have been posted so check out our <a href="https://2010.igem.org/wiki/Team:DTU-Denmark/Gallery">picture gallery</a>!<br>
+<p>Pictures have been posted so check out our <a href="https://2010.igem.org/Team:DTU-Denmark/Gallery">picture gallery</a>!<br>
 <br>
 </p>
@@ Line 216: / Line 274: @@
 <font face="arial" size="1">
-  Comments or questions to the team? Please <a href="mailto:igem2010@bio.dtu.dk" CLASS=email>Email us</a> -- Comments of questions to webmaster? Please <a href="mailto:igem2010@bio.dtu.dk" CLASS=email>Email us</a>
+  Comments or questions to the team? Please <a href="mailto:igem2010@bio.dtu.dk" CLASS=email>Email us</a>
 </font>