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Team:Valencia/Notebook/July
From 2010.igem.org
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==WETLab meeting== | ==WETLab meeting== | ||
| - | We start to put | + | We start to put together all the thing, to begin working in the Lab!! |
| - | + | We also made plans for the future. | |
| - | We prepared the | + | We prepared the culture medium for growing our yeasts. |
[[Image:valencia_gabi.jpg|Gabi with the super yeast culture medium|200px|thumb]] | [[Image:valencia_gabi.jpg|Gabi with the super yeast culture medium|200px|thumb]] | ||
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=July 9th= | =July 9th= | ||
| - | We filled up the petri | + | We filled up the petri dishes with the yeast liquid culture medium. |
| - | Then we planted the yeasts | + | Then we planted the yeasts onto the petri dishes. |
We prepared an overnight culture at 37ºC into a stove with the bacteria having the plasmids pG1 and pLZ in order to purificate them next Monday. | We prepared an overnight culture at 37ºC into a stove with the bacteria having the plasmids pG1 and pLZ in order to purificate them next Monday. | ||
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Then we carried out a digestion and the following electrophoresis to verify whether we had the constructions in the plasmids and to evaluate their concentration. | Then we carried out a digestion and the following electrophoresis to verify whether we had the constructions in the plasmids and to evaluate their concentration. | ||
We stored the non-digested plasmids in the refrigerator (we want to use it later on) | We stored the non-digested plasmids in the refrigerator (we want to use it later on) | ||
| - | We made a liquid culture of E. coli with the LEA gene, and puted it into the stove at 37ºC. | + | We made a liquid culture of ''E. coli'' with the LEA gene, and puted it into the stove at 37ºC. |
| - | We recieved a filter paper soaked with a plasmid from Kausik Li containing an Aplysia prion fused with the GR domain. Then we put the paper containing the plasmid in water in order to transform and clone it in E. coli later on. | + | We recieved a filter paper soaked with a plasmid from Kausik Li containing an ''Aplysia'' prion fused with the GR domain. Then we put the paper containing the plasmid in water in order to transform and clone it in ''E. coli'' later on. |
=July 13th= | =July 13th= | ||
Revision as of 11:18, 13 July 2010
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Contents |
July 8th
WETLab meeting
We start to put together all the thing, to begin working in the Lab!! We also made plans for the future.
We prepared the culture medium for growing our yeasts.
Social Event
We got together in Serranos Towers, to chill out a litle bit after the "hard work" in the lab.
July 9th
We filled up the petri dishes with the yeast liquid culture medium. Then we planted the yeasts onto the petri dishes. We prepared an overnight culture at 37ºC into a stove with the bacteria having the plasmids pG1 and pLZ in order to purificate them next Monday.
July 10th
We transferred the bacterial culture from the stove (37ºC) to the fridge (4ºC).
July 11th
Spain won the World cup!!!!
July 12th
We made a miniprep to purify the plasmids pG1 and pLZ. Then we carried out a digestion and the following electrophoresis to verify whether we had the constructions in the plasmids and to evaluate their concentration. We stored the non-digested plasmids in the refrigerator (we want to use it later on) We made a liquid culture of E. coli with the LEA gene, and puted it into the stove at 37ºC. We recieved a filter paper soaked with a plasmid from Kausik Li containing an Aplysia prion fused with the GR domain. Then we put the paper containing the plasmid in water in order to transform and clone it in E. coli later on.
July 13th
We carried out a miniprep to purify the pM2 plasmid containing the LEA gene. Then we carried out a digestion and the following electrophoresis to verify whether we had the constructions in the plasmids and to evaluate their concentration.
