Team:Korea U Seoul/Parts

From 2010.igem.org

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=='''Part:BBa_K400000'''==
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*Designed by Jonghwa Kim  Group: iGEM10_Korea_U_Seoul  (2010-10-21) composite heavy metal-detector(Zn, Cd, As)
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*It is a composite part of heavy metal-detecting promoters and reporter genes.
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:PyodA-mAAA(BBa_K400628)----PzntA-RFP(BBa_E0040)----ParsR-GFP(BBa_J04450)----
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:It detects cadmium, zinc, and arsenic in the media and gives brown, red, and green color. This is our final product to put in a
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:capsule in order to be customized.
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=='''Part:BBa_K400628'''==
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*Designed by Seobeom Heo  Group: iGEM10_Korea_U_Seoul  (2010-10-19)
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*mAAA (EcoRI site mutated Aryl Acyl-Amidase)
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*Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds. The most typical reaction is the hydrolysis :of an anilide, producing a carboxylate and aniline, which is reversible. When a p-acetaminophenol was reacted with AAA, the product gave :a purple-red color. As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it :was grown on solid media enriched with p-acetaminophenol.Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter. In our project, we put it :downstream of cadmium-dependent promoter to detect any cadmium present in a solution.

Latest revision as of 01:15, 28 October 2010

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Contents

Parts

New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.

<groupparts>iGEM010 Korea_U_Seoul</groupparts>

Character

Part:BBa_K400000

  • Designed by Jonghwa Kim Group: iGEM10_Korea_U_Seoul (2010-10-21) composite heavy metal-detector(Zn, Cd, As)
  • It is a composite part of heavy metal-detecting promoters and reporter genes.
PyodA-mAAA(BBa_K400628)----PzntA-RFP(BBa_E0040)----ParsR-GFP(BBa_J04450)----
It detects cadmium, zinc, and arsenic in the media and gives brown, red, and green color. This is our final product to put in a
capsule in order to be customized.


Part:BBa_K400628

  • Designed by Seobeom Heo Group: iGEM10_Korea_U_Seoul (2010-10-19)
  • mAAA (EcoRI site mutated Aryl Acyl-Amidase)
  • Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds. The most typical reaction is the hydrolysis :of an anilide, producing a carboxylate and aniline, which is reversible. When a p-acetaminophenol was reacted with AAA, the product gave :a purple-red color. As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it :was grown on solid media enriched with p-acetaminophenol.Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter. In our project, we put it :downstream of cadmium-dependent promoter to detect any cadmium present in a solution.