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Team:Queens-Canada/21 June 2010
From 2010.igem.org
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| - | + | {{:Team:Queens-Canada/head}} | |
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| + | {{:Team:Queens-Canada/head}} | ||
| + | <h2>June 21, 2010</h2> | ||
| + | On this day... | ||
| + | |||
| + | * We got the wiki up and going! | ||
| + | * We ran a crazy gel and cut it up a whole bunch. | ||
| + | |||
| + | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
| + | Hao | ||
| + | |||
| + | '''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-7'''::'''eGFP''' | ||
| + | |||
| + | Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V. | ||
| + | |||
| + | <h3>[[Team:Queens-Canada/protocols#Gel Extraction|Gel Extraction]]</h3> | ||
| + | Yuli and Hao | ||
| + | |||
| + | '''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-7'''::'''eGFP''' | ||
| + | |||
| + | Products stored at -20 °C. | ||
| + | |||
| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao and Yuli | ||
| + | |||
| + | Practiced injections with water. | ||
| + | |||
| + | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 01:08, 28 October 2010
June 21, 2010
On this day...
- We got the wiki up and going!
- We ran a crazy gel and cut it up a whole bunch.
Gel Electrophoresis
Hao
pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP
Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.
Gel Extraction
Yuli and Hao
pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP
Products stored at -20 °C.
Microinjection
Hao and Yuli
Practiced injections with water.
