Team:Stockholm/Results/BioBricks

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{{Stockholm/Results}}
{{Stockholm/Results}}
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{|align="right"
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==BioBricks==
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===''Trans''-Activating Transcriptional Activator (TAT)===
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==Cell-penetrating peptides==
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====[http://partsregistry.org/Part:BBa_K380000 BBa_K380000]: Standard part====
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[[image:TAT_BioBrick.png]]
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:'''Part name:''' TAT cell-penetrating peptide<br />
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:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#TAT_cell_penetrating_peptide_.28TAT.29|more]])<br />
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:Cpp1 premix.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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<br />
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====[http://partsregistry.org/Part:BBa_K380001 BBa_K380001]: N-part====
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[[image:NTAT_BioBrick.png]]
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 +
:'''Part name:''' N-part TAT cell-penetrating peptide<br />
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:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#TAT_cell_penetrating_peptide_.28TAT.29|more]])<br />
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_12_premix.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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 +
<br />
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----
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<br />
===Low Molecular Weight Protamine (LMWP)===
===Low Molecular Weight Protamine (LMWP)===
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'''BBa_K38xxxx''' (Standard part)
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====[http://partsregistry.org/Part:BBa_K380002 BBa_K380002]: Standard part)====
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[[image:LMWP_BioBrick.png]]
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Sequence (fasta)
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:'''Part name:''' LMWP cell-penetrating peptide<br />
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:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Low_molecular_weight_protamine_.28LMWP.29|more]])<br />
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:Cpp2 premix.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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<br />
-
'''BBa_K38xxxx''' (N-part)
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====[http://partsregistry.org/Part:BBa_K380003 BBa_K380003]: N-part====
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[[image:nLMWP_BioBrick.png]]
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Sequence (fasta)
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:'''Part name:''' N-part LMWP cell-penetrating peptide<br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Low_molecular_weight_protamine_.28LMWP.29|more]])<br />
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_11_premix.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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===Transportan 10 (Tra10)===
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<br />
 +
----
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<br />
-
'''BBa_K38xxxx''' (Standard part)
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===Transportan 10 (Tp10)===
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Sequence (fasta)
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====[http://partsregistry.org/Part:BBa_K380004 BBa_K380004]: Standard part====
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[[image:Tp10_BioBrick.png]]
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'''BBa_K38xxxx''' (N-part)
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:'''Part name:''' Tp10 cell-penetrating peptide<br />
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:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Transportan_10_.28Tp10.29|more]])<br />
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Cpp12 premix.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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<br />
-
Sequence (fasta)
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====[http://partsregistry.org/Part:BBa_K380005 BBa_K380005]: N-part====
 +
[[image:nTp10_BioBrick.png]]
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===''Trans''-Activating Transcriptional Activator (TAT)===
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:'''Part name:''' N-part Tp10 cell-penetrating peptide<br />
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:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Transportan_10_.28Tp10.29|more]])<br />
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_5_premix.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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 +
<br />
 +
----
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<br />
 +
 
 +
===[http://partsregistry.org/Part:BBa_K380006 BBa_K380006]: Human Basic Fibroblast Growth Factor (bFGF)===
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[[image:BFGF_BioBrick.png]]
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:'''Part name:''' Human basic fibroblast growth factor, bFGF<br />
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:'''Property:''' Growth factor ([[Team:Stockholm/Project_Idea/Proteins#Human_basic_fibroblast_growth_factor_.28bFGF.29|more]])<br />
 +
:'''Edited nucleotide(s):''' nt341 C &rarr; T
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:'''Removed restr. site(s):''' AgeI
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.bFGF_VR_premix_fasta.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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TAT is a cell-penetrating peptide that may be used in fusions with full-length proteins to create transduction proteins with the ability to permeate the lipid bilayer of various cell types.
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<br />
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----
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<br />
-
Purified full-length TAT fusion proteins expressed in ''Escherichia coli'' have been shown to successfully translocate into several human cell types, including all cells found in whole blood, as well as bone marrow stem cells and osteoblasts, while still retaining the fused protein's activity ([http://www.ncbi.nlm.nih.gov/pubmed/9846587 Nagahara ''et al.'' 1998]). The mechanism for transduction over the bilipid membrane is still a matter of debate, but has been suggested to occur through macropinocytosis, a specialized form of endocytosis ([http://www.ncbi.nlm.nih.gov/pubmed/17913584 Gump and Dowdy, 2007]).
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===[http://partsregistry.org/Part:BBa_K380007 BBa_K380007]: Superoxide dismutase 1 (SOD1)===
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[[image:SOD1_BioBrick.png]]
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====Standard part====
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:'''Part name:''' Superoxide dismutase 1 protein<br />
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:'''Property:''' Catalyzes the reaction O<sup>-</sup><sub>2</sub> + O<sup>-</sup><sub>2</sub> + 2H<sup>+</sup> → H<sub>2</sub>O<sub>2</sub> + O<sub>2</sub> ([[Team:Stockholm/Project_Idea/Proteins#Superoxide_dismutase_1_.28SOD1.29|more]])<br />
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:'''Edited nucleotide(s):''' nt331 A &rarr; G
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:'''Removed restr. site(s):''' PfeI
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.SOD_VR_premix_fasta.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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'''Part name:''' TAT cell-penetrating peptide<br />
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<br />
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'''Registry name:''' [http://partsregistry.org/Part:BBa_K380000 BBa_K380000]<br />
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----
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'''Sequencing result:''' <span style="color:green;">Verified</span> (fasta)<br />
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<br />
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'''Functionality:''' <span style="color:red;">Not verified</span>
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-
====Standard part====
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===[http://partsregistry.org/Part:BBa_K380008 BBa_K380008]: Yeast Copper Chaperone (yCCS)===
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[[image:YCCS_BioBrick.png]]
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'''Part name:''' N-part TAT cell-penetrating peptide<br />
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:'''Part name:''' Yeast copper chaperone protein<br />
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'''Registry name:''' [http://partsregistry.org/Part:BBa_K380000 BBa_K380001]<br />
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:'''Property:''' Mediates the delivery of copper ions to SOD1 ([[Team:Stockholm/Project_Idea/Proteins#Yeast_copper_chaperon_.28yCCS.29|more]])<br />
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'''Sequencing result:''' <span style="color:green;">Verified</span> (fasta)<br />
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:'''Edited nucleotide(s):''' nt257 T &rarr; C
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'''Functionality:''' <span style="color:red;">Not verified</span>
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:'''Removed restr. site(s):''' EcoRI
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.yCCS_VR_premix_fasta.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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==Proteins==
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<br />
 +
----
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<br />
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===Immunoglobulin G protease (IdeS)===
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===[http://partsregistry.org/Part:BBa_K380009 BBa_K380009]: Protein A Z-domain (PtA-Z)===
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[[image:ProteinA_BioBrick.png]]
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===Protein A Z-domain (ProtA-Z)===
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:'''Part name:''' Protein A Z-domain<br />
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:'''Property:''' Immunoglobulin Fc region binding ([[Team:Stockholm/Project_Idea/Proteins#Protein_A.2C_z_domain|more]])<br />
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:'''Edited nucleotide(s):''' -
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:'''Removed restr. site(s):''' -
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.ProtA VR premix fasta.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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===Superoxide dismutase (SOD)===
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<br />
 +
----
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<br />
-
====Yeast Copper Chaperone (yCCS)====
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===[http://partsregistry.org/Part:BBa_K380010 BBa_K380010]: Immunoglobulin G protease (IdeS)===
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[[image:IdeS_BioBrick.png]]
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===Basic Fibroblast Growth Factor (bFGF)===
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:'''Part name:''' Immunoglobulin G protease (IdeS)<br />
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:'''Property:''' Immunoglobulin G protease ([[Team:Stockholm/Project_Idea/Proteins#IgG_protease_.28IdeS.29|more]])<br />
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:'''Edited nucleotide(s):''' -
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:'''Removed restr. site(s):''' -
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:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.IgGp_VR_premix_fasta.txt|fasta]])<br />
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:'''Works:''' <span style="color:red;">Not known</span>
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|}
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{{Stockholm/Footer}}

Latest revision as of 01:03, 28 October 2010

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↳   BioBricks Assemblies Medals criteria


Contents

BioBricks

Trans-Activating Transcriptional Activator (TAT)

[http://partsregistry.org/Part:BBa_K380000 BBa_K380000]: Standard part

TAT BioBrick.png

Part name: TAT cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known


[http://partsregistry.org/Part:BBa_K380001 BBa_K380001]: N-part

NTAT BioBrick.png

Part name: N-part TAT cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known



Low Molecular Weight Protamine (LMWP)

[http://partsregistry.org/Part:BBa_K380002 BBa_K380002]: Standard part)

LMWP BioBrick.png

Part name: LMWP cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known


[http://partsregistry.org/Part:BBa_K380003 BBa_K380003]: N-part

NLMWP BioBrick.png

Part name: N-part LMWP cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known



Transportan 10 (Tp10)

[http://partsregistry.org/Part:BBa_K380004 BBa_K380004]: Standard part

Tp10 BioBrick.png

Part name: Tp10 cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known


[http://partsregistry.org/Part:BBa_K380005 BBa_K380005]: N-part

NTp10 BioBrick.png

Part name: N-part Tp10 cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known



[http://partsregistry.org/Part:BBa_K380006 BBa_K380006]: Human Basic Fibroblast Growth Factor (bFGF)

BFGF BioBrick.png

Part name: Human basic fibroblast growth factor, bFGF
Property: Growth factor (more)
Edited nucleotide(s): nt341 C → T
Removed restr. site(s): AgeI
Sequenced: Yes (fasta)
Works: Not known



[http://partsregistry.org/Part:BBa_K380007 BBa_K380007]: Superoxide dismutase 1 (SOD1)

SOD1 BioBrick.png

Part name: Superoxide dismutase 1 protein
Property: Catalyzes the reaction O-2 + O-2 + 2H+ → H2O2 + O2 (more)
Edited nucleotide(s): nt331 A → G
Removed restr. site(s): PfeI
Sequenced: Yes (fasta)
Works: Not known



[http://partsregistry.org/Part:BBa_K380008 BBa_K380008]: Yeast Copper Chaperone (yCCS)

YCCS BioBrick.png

Part name: Yeast copper chaperone protein
Property: Mediates the delivery of copper ions to SOD1 (more)
Edited nucleotide(s): nt257 T → C
Removed restr. site(s): EcoRI
Sequenced: Yes (fasta)
Works: Not known



[http://partsregistry.org/Part:BBa_K380009 BBa_K380009]: Protein A Z-domain (PtA-Z)

ProteinA BioBrick.png

Part name: Protein A Z-domain
Property: Immunoglobulin Fc region binding (more)
Edited nucleotide(s): -
Removed restr. site(s): -
Sequenced: Yes (fasta)
Works: Not known



[http://partsregistry.org/Part:BBa_K380010 BBa_K380010]: Immunoglobulin G protease (IdeS)

IdeS BioBrick.png

Part name: Immunoglobulin G protease (IdeS)
Property: Immunoglobulin G protease (more)
Edited nucleotide(s): -
Removed restr. site(s): -
Sequenced: Yes (fasta)
Works: Not known





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

Retrieved from "http://2010.igem.org/Team:Stockholm/Results/BioBricks"